Team:AUC Turkey/Project/Results

BBa_K727001 MMT BBa_K727002 DMT BBa_K727003 TMT BBa_K727004 FMO BBa_K727005 GPPS BBa_K72700 GS

6 tane resim

We succesfully transformated these 6 parts into E.coli’s TOP10 strains as you can see above. Then we did overnight incubation to the colonies in LB Media and afterwards they were isolated. The acquiring of the DNA was a success. (Spektrofotometre bilgileri eklenecek.) Lastly, the parts were cut with the appropriate restriction enzymes and electrophorased. (UV Sonuçları eklenecek.) As it can be seen, the bends on the images overlap with the indicated lengths of our biobricks which proves that we succesfully cloned our parts into E.coli.

The parts we assembled: BBa_K727007 Rose Odor Generator Device BBa_K727008 Fish Odor Generator-Component 1 BBa_K727009 Fish Odor Generator-Component 2 BBa_K727011 Fish Odor Generator-Component 3 BBa_K727727 FMO3 Generator BBa_K727777 FMO Generator- Complete System After confirming the basic parts, we assembled the parts listed above in the appropriate order. The parts were then transformated into E.coli’s TOP10 strain. We did this by adding constituve promoter and GFP to see green colonies which would in the cate both our GFP and Down Stream BioBrick has been synthesized. For example the image at the below shows that BBa_727005 is most probably produced. Green Picture

After that we inchubated our colonies in LB Broth Media then we isolated genes with high yield.

OD We cut our genes with appropiate restriction enzymes and exposed them to electrophoresis. The images below prove that we clonned our BioBricks successfully. Electro UV Green Picture

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