Team:Sharon MA Aquila/Project

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Revision as of 19:15, 3 April 2012 by Hannahb (Talk | contribs)


Parts

  • [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria Lactobacillus delbrueckii subsp. bulgaricus] (from ATCC)
  • [http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)

Materials

  • Culturing:
    • [http://www.bd.com/ds/productCenter/288110.asp MRS broth]
    • [http://www.bd.com/ds/productCenter/288210.asp MRS agar]
  • Bacterial Transformation:
    • Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
    • Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
    • Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
    • Gene Pulser and Pulse Controller Electrophoration apparatus
    • Gaspak or method to generate anaerobic conditions


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