Team:Sharon MA Aquila/Project

From 2012hs.igem.org


Parts

Our pH sensor

Materials

  • Culturing:
  • Bacterial Transformation:
    • Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
    • Erythromycin (for selection)
    • Gene Pulser and Pulse Controller Electrophoration apparatus
    • Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
  • Miniprep
    • TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
    • 10mg/ml lysozyme
    • 70U/ml mutanolysin