"
Team:Sharon MA Aquila/Project
From 2012hs.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
**Gene Pulser and Pulse Controller Electrophoration apparatus | **Gene Pulser and Pulse Controller Electrophoration apparatus | ||
**Gaspak or method to generate anaerobic conditions | **Gaspak or method to generate anaerobic conditions | ||
| + | *'''Miniprep''' | ||
| + | **TES(50mM Tris[pH 7] | ||
| + | **20mM EDTA, 0.2 mM sucrose) | ||
| + | **10mg/ml lysozyme | ||
| + | **70U/ml mutanolysin | ||
<forum_subtle /> | <forum_subtle /> | ||
Revision as of 19:17, 3 April 2012
Parts
- [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria Lactobacillus delbrueckii subsp. bulgaricus] (from ATCC)
- [http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)
Materials
- Culturing:
- [http://www.bd.com/ds/productCenter/288110.asp MRS broth]
- [http://www.bd.com/ds/productCenter/288210.asp MRS agar]
- Bacterial Transformation:
- Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
- Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
- Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
- Gene Pulser and Pulse Controller Electrophoration apparatus
- Gaspak or method to generate anaerobic conditions
- Miniprep
- TES(50mM Tris[pH 7]
- 20mM EDTA, 0.2 mM sucrose)
- 10mg/ml lysozyme
- 70U/ml mutanolysin
