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Team:Sharon MA Aquila/Project
From 2012hs.igem.org
(Difference between revisions)
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{{Aquila}} | {{Aquila}} | ||
| - | ===Parts | + | ===Parts=== |
*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC) | *[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC) | ||
*[http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry) | *[http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry) | ||
| - | *''Culturing'' | + | ===Materials=== |
| + | *'''Culturing:''' | ||
**[http://www.bd.com/ds/productCenter/288110.asp MRS broth] | **[http://www.bd.com/ds/productCenter/288110.asp MRS broth] | ||
**[http://www.bd.com/ds/productCenter/288210.asp MRS agar] | **[http://www.bd.com/ds/productCenter/288210.asp MRS agar] | ||
| + | *'''Bacterial Transformation:''' | ||
| + | **Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) | ||
| + | **Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2) | ||
| + | **Erythromycin, Chloramphenicol, other antibiotics may be needed for selection | ||
| + | **Gene Pulser and Pulse Controller Electrophoration apparatus | ||
| + | **Gaspak or method to generate anaerobic conditions | ||
| + | |||
<forum_subtle /> | <forum_subtle /> | ||
Revision as of 19:15, 3 April 2012
Parts
- [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria Lactobacillus delbrueckii subsp. bulgaricus] (from ATCC)
- [http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)
Materials
- Culturing:
- [http://www.bd.com/ds/productCenter/288110.asp MRS broth]
- [http://www.bd.com/ds/productCenter/288210.asp MRS agar]
- Bacterial Transformation:
- Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
- Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
- Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
- Gene Pulser and Pulse Controller Electrophoration apparatus
- Gaspak or method to generate anaerobic conditions
