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Team:Sharon MA Aquila/Project
From 2012hs.igem.org
(Difference between revisions)
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*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001) | *[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001) | ||
*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006) | *[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006) | ||
| - | *[http://partsregistry.org/Part:BBa_K128007 Plasmid] to confer Erythromycin resistance to bacteria | + | *[http://partsregistry.org/Part:BBa_K128007 Plasmid] to confer Erythromycin resistance to bacteria (from the Parts Registry BBa_K128007) |
===Materials=== | ===Materials=== | ||
Revision as of 01:01, 4 April 2012
Parts
- [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria Lactobacillus delbrueckii subsp. bulgaricus] (from ATCC)
- [http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)
- [http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)
- [http://partsregistry.org/Part:BBa_K128007 Plasmid] to confer Erythromycin resistance to bacteria (from the Parts Registry BBa_K128007)
Materials
- Culturing:
- [http://www.bd.com/ds/productCenter/288110.asp MRS broth]
- [http://www.bd.com/ds/productCenter/288210.asp MRS agar]
- Bacterial Transformation:
- Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
- Erythromycin (for selection)
- Gene Pulser and Pulse Controller Electrophoration apparatus
- Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
- Miniprep
- TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
- 10mg/ml lysozyme
- 70U/ml mutanolysin
