Team:Sharon MA Aquila/Project

From 2012hs.igem.org

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===Parts===
===Parts===
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*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)
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[http://http://partsregistry.org/Part:BBa_K116001 Our pH sensor]
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*[http://partsregistry.org/Part:BBa_K652001 RiAFP Part] (from the Parts Registry BBa_K652001)
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*[http://partsregistry.org/Part:BBa_K128006 LacS Promoter] (from the Parts Registry BBa_K128006)
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===Materials===
===Materials===
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*'''Bacterial Transformation:'''
*'''Bacterial Transformation:'''
**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
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**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
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**Erythromycin (for selection)
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**Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
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**Gene Pulser and Pulse Controller Electrophoration apparatus
**Gene Pulser and Pulse Controller Electrophoration apparatus
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**Gaspak or method to generate anaerobic conditions
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**Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
*'''Miniprep'''
*'''Miniprep'''
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**TES(50mM Tris[pH 7]
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**TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
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**20mM EDTA, 0.2 mM sucrose)
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**10mg/ml lysozyme
**10mg/ml lysozyme
**70U/ml mutanolysin
**70U/ml mutanolysin

Latest revision as of 19:37, 21 May 2013


Parts

[http://http://partsregistry.org/Part:BBa_K116001 Our pH sensor]

Materials

  • Culturing:
    • [http://www.bd.com/ds/productCenter/288110.asp MRS broth]
    • [http://www.bd.com/ds/productCenter/288210.asp MRS agar]
  • Bacterial Transformation:
    • Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
    • Erythromycin (for selection)
    • Gene Pulser and Pulse Controller Electrophoration apparatus
    • Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
  • Miniprep
    • TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
    • 10mg/ml lysozyme
    • 70U/ml mutanolysin


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