Team:Sharon MA Aquila/Project
From 2012hs.igem.org
Parts
- Lactobacillus delbrueckii subsp. bulgaricus (from ATCC)
- RiAFP Part (from the Parts Registry BBa_K652001)
- LacS Promoter (from the Parts Registry BBa_K128006)
- Plasmid to confer Erythromycin resistance to bacteria
Materials
- Culturing:
- Bacterial Transformation:
- Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
- Erythromycin (for selection)
- Gene Pulser and Pulse Controller Electrophoration apparatus
- Gaspak or method to generate anaerobic conditions (airtight box with lit candles)
- Miniprep
- TES(50mM Tris[pH 7], 20mM EDTA, 0.2 mM sucrose)
- 10mg/ml lysozyme
- 70U/ml mutanolysin