Team:Sharon MA Aquila/Project

From 2012hs.igem.org

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**Gene Pulser and Pulse Controller Electrophoration apparatus
**Gene Pulser and Pulse Controller Electrophoration apparatus
**Gaspak or method to generate anaerobic conditions
**Gaspak or method to generate anaerobic conditions
 +
*'''Miniprep'''
 +
**TES(50mM Tris[pH 7]
 +
**20mM EDTA, 0.2 mM sucrose)
 +
**10mg/ml lysozyme
 +
**70U/ml mutanolysin
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Revision as of 19:17, 3 April 2012


Parts

Materials

  • Culturing:
  • Bacterial Transformation:
    • Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
    • Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
    • Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
    • Gene Pulser and Pulse Controller Electrophoration apparatus
    • Gaspak or method to generate anaerobic conditions
  • Miniprep
    • TES(50mM Tris[pH 7]
    • 20mM EDTA, 0.2 mM sucrose)
    • 10mg/ml lysozyme
    • 70U/ml mutanolysin


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