Team:Sharon MA Aquila/Project

From 2012hs.igem.org

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{{Aquila}}
{{Aquila}}
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===Parts:===
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===Parts===
*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)
*[http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11842&Template=bacteria ''Lactobacillus delbrueckii'' subsp. ''bulgaricus''] (from ATCC)
*[http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)
*[http://partsregistry.org/Part:BBa_K652001 RiAFP part] (from the Parts Registry)
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*''Culturing''
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===Materials===
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*'''Culturing:'''
**[http://www.bd.com/ds/productCenter/288110.asp MRS broth]
**[http://www.bd.com/ds/productCenter/288110.asp MRS broth]
**[http://www.bd.com/ds/productCenter/288210.asp MRS agar]  
**[http://www.bd.com/ds/productCenter/288210.asp MRS agar]  
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*'''Bacterial Transformation:'''
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**Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
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**Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
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**Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
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**Gene Pulser and Pulse Controller Electrophoration apparatus
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**Gaspak or method to generate anaerobic conditions
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Revision as of 19:15, 3 April 2012


Parts

Materials

  • Culturing:
  • Bacterial Transformation:
    • Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6)
    • Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2)
    • Erythromycin, Chloramphenicol, other antibiotics may be needed for selection
    • Gene Pulser and Pulse Controller Electrophoration apparatus
    • Gaspak or method to generate anaerobic conditions


<forum_subtle />
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