Team:AUC Turkey/Project/Assembly

From 2012hs.igem.org

Sponsors


Assembly

BBa_K747004

AUC Turkey BBa 747004.png

FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

BBa_K747747

AUC Turkey BBa 747747.png

FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.

BBa_K747001

AUC Turkey BBa 747001.png

MMT (monomethylamine transferase) catalyses the first reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

BBa_K747002

AUC Turkey BBa 747002.png

DMT (dimethylamine transferase) takes part in the second reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

BBa_K747003

AUC Turkey BBa 747003.png

TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

BBa_K747777

AUC Turkey BBa 747777.png

TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a negative feedback response with the help of producing LuxR and HSL and activating the promoter with them.

BBa_K747005

AUC Turkey BBa 747005.png

GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production. The promoter is attached into the part with the aim of easier regulation.

BBa_K747006

AUC Turkey BBa 747006.png

GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. The promoter is attached into the part with the aim of easier regulation.

BBa_K747700

AUC Turkey BBa 747700.png

GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.