Team:Lethbridge Canada/Notebook
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'''May 9, 2012: Restriction of Parts''' | '''May 9, 2012: Restriction of Parts''' | ||
- | [[Image:May_9th_restriction. | + | [[Image:May_9th_restriction.JPG|center|740px]] |
Master mix was prepared by mixing the ingredients together in order, as shown above. 20µL worth of master mix was combined with 5µL of plasmid DNA (1-5). | Master mix was prepared by mixing the ingredients together in order, as shown above. 20µL worth of master mix was combined with 5µL of plasmid DNA (1-5). | ||
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-B0034 pSB1A2 | -B0034 pSB1A2 | ||
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+ | '''May 10,2012: Restriction of Parts''' | ||
+ | [[Image:May_10th_restriction.JPG|center|740px]] | ||
+ | Master mix was prepared by mixing the ingredients together in order, as shown above. 20µL worth of master mix was combined with 5µL of plasmid DNA (1-7). | ||
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+ | -J23100 (J61002) | ||
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+ | -J23100 (J61002) | ||
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+ | -B0034 (pSB1A3) | ||
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+ | -B0034 (pSB1A3) | ||
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+ | -E1010 (pSB2K3) | ||
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+ | -E1010 (pSB2k3) | ||
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+ | -K093008 (pSB1A2) | ||
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+ | Incubated at 37˚C for 30min then stored in -20˚C. | ||
==June== | ==June== |
Revision as of 22:15, 29 May 2012
Home | The Team | The Project | Results | Human Practices | Notebook | Safety |
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NotebookMarchBrainstormingProject ideas:
Kill Switches (engineering bacteria to undergo induced or programmed cell death in order to control the organism) The chosen projectAfter dividing in to a few groups based on which project was preferred, each group made a presentation on the proposed project. In the end, we decided to go with the glucose sensing project. Project outlinePossible things to consider Glucose and insulin Immune response Kill switch Delivery system AprilWe divided the team into sub-groups. Each group assumed responsibility for a different aspect of the project. Sub-groups: -Wiki and Mascot Design -Biosafety -Human Practices Wiki and Mascot Design: This group is responsible to obtaining and uploading all necessary information to the wiki. This includes team pictures and biographies, experiments and results, and the projects of the other sub-groups. This will be a priority over mascot design. Biosafety: This group must demontrate the safety protocols that the Lethbridge team practices in the lab. Human Practices: This group is responsible for letting the poublic know what our team is and what we are doing. This can be done creatively. The point it to raise awareness for iGEM, synthetic biology and diabetes. MayMay 8, 2012: Tansformation of Parts from Kit Plate -B0034 in pSB1A2 -K093005 in pSB1A2 -J23100 in J61002 (Amp) -E1010 in pSB2K3 Overnight cultures were also made of: -pMA-T K33109 (HST-1) -pSB1AK3 B0014
Master mix was prepared by mixing the ingredients together in order, as shown above. 20µL worth of master mix was combined with 5µL of plasmid DNA (1-5).
pSB1AK3 BBa_B0014 Incubated at 37˚C for 30mins then stored at -20˚C.
-E1010 pSB2K3 -J23100 J61002 (Amp) -K93005 pSB1A2 -B0034 pSB1A2
Master mix was prepared by mixing the ingredients together in order, as shown above. 20µL worth of master mix was combined with 5µL of plasmid DNA (1-7). -J23100 (J61002) -J23100 (J61002) -B0034 (pSB1A3) -B0034 (pSB1A3) -E1010 (pSB2K3) -E1010 (pSB2k3) -K093008 (pSB1A2) Incubated at 37˚C for 30min then stored in -20˚C. June
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