Team:NC School of Sci Math

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have the following information on your wiki:
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:NC_School_of_Sci_Math_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:NC_School_of_Sci_Math | Team NC_School_of_Sci_Math]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&division=high_school&team_name=NC_School_of_Sci_Math Official Team Profile]
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===Team===
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Tell us about your team, your school!
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===Project===
 
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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===Attributions===
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Who worked on what?
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<li class="first"><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math" accesskey="1" title="">Home</a></li>
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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/teamdescription" accesskey="2" title="">Meet the Team</a></li>
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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/projectdescription" accesskey="3" title="">Project Description</a></li>
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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/safetyprocedure" accesskey="4" title="">Safety</a></li>
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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/notebooks" accesskey="5" title="">Lab Notebook</a></li>
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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/sponsors" accesskey="5" title="">Sponsors</a></li>
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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/humanpractices" accesskey="6" title="">Human Practices</a></li>
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===Human Practices===
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<h2 class="title"><a href="#">Welcome to the NCSSM iGEM Wiki</a></h2>
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<h2>NCSSM</h2>
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<p>The North Carolina School of Science and Mathematics is residential public high school for highly gifted 11th and 12th graders in the state of North Carolina. Students at NCSSM regularly take advantage of the schools research opportunities. NCSSM iGEM is the latest research opportunity to these students!</p>
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<h2>Useful Links</h2>
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<li><a href="http://www.ncssm.edu/">NCSSM</a></li>
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<li><a href="http://www.bowmanbrockman.org/ncssm/">Bowman Brockman</a></li>
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<li><a href="http://www.geneoracle.com/">Gene Oracle</a></li>
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<li><a href="http://www.synberc.org/">SynBERC</a></li>
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<li><a href="http://pubs.acs.org/journal/asbcd6">ACS Synethetic Biology</a></li>
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===Fun!===
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What was your favorite team snack?? Have a picture of your team mascot?
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===Abstract===
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Silver nanoparticles (AgNP’s) have a variety of beneficial health effects but can be detrimental to both human and environmental health in excessive quantities. As a result, NCSSM iGEM has attempted to develop an inexpensive biosensor for detecting AgNP’s in vivo. This novel AgNP biosensor utilizes ACE1, a silver binding transcription factors from S. cerevisiae (yeast), in an E. coli chassis. In the biosensor’s synthetic gene network, a constitutive promoter upstream of ACE1 ensures continuous expression of the ACE1 protein. In the presence of AgNP’s, ACE1 activates the CUP1 promoter which has been placed upstream of a Green Fluorescent Protein gene. Subsequently, exposure of the E. coli biosensor to AgNP’s will result in high levels of GFP expression. Network success was confirmed in vivo by spreading the biosensor on plates containing varying concentrations of AgNP’s. However, due to the antimicrobial effects of AgNP’s, network success under high AgNP concentrations remains unconfirmed.
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Latest revision as of 02:27, 17 June 2012

Welcome to the NCSSM iGEM Wiki

Abstract

Silver nanoparticles (AgNP’s) have a variety of beneficial health effects but can be detrimental to both human and environmental health in excessive quantities. As a result, NCSSM iGEM has attempted to develop an inexpensive biosensor for detecting AgNP’s in vivo. This novel AgNP biosensor utilizes ACE1, a silver binding transcription factors from S. cerevisiae (yeast), in an E. coli chassis. In the biosensor’s synthetic gene network, a constitutive promoter upstream of ACE1 ensures continuous expression of the ACE1 protein. In the presence of AgNP’s, ACE1 activates the CUP1 promoter which has been placed upstream of a Green Fluorescent Protein gene. Subsequently, exposure of the E. coli biosensor to AgNP’s will result in high levels of GFP expression. Network success was confirmed in vivo by spreading the biosensor on plates containing varying concentrations of AgNP’s. However, due to the antimicrobial effects of AgNP’s, network success under high AgNP concentrations remains unconfirmed.

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