Notebook

From 2012hs.igem.org

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[[File:toolbar_team.png|140px|link=Team|Team]] [[File:toolbar_project.png|140px|link=Project|Project]] [[File:toolbar_notebook.png|140px|link=Notebook|Notebook]] [[File:toolbar_Results.png|140px|link=Results|Results]]
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='''Notebook'''=
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[[File:toolbar_Safety.png|140px|link=Safety|Safety]] [[File:toolbar_Sponsors.png|140px|link=Sponsors|Sponsors]]
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==March==
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===Brainstorming ===
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Project ideas: <br>
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''Pests'' <br>
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·      Bacterial Pest attractor (engineer bacteria to produce a substance, such as a pheromone or smell, that attracts and kills pests such as insects) <br>
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·      Natural pesticides (engineer bacteria to produce a substance that repels or kills pests, such as those that harm crops, that can later be implemented into plants) <br>
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''Water/Environment:'' <br>
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·      Desalination of water (engineering bacteria to get rid of salt in salt water in order to make drinkable water from sea water) <br>
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·      Getting rid of estrogen mimicking compounds in water (engineering bacteria to degrade them or sequester them) <br>
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·      Waste treatment (engineering bacteria that can be integrated into waste water treatment) <br>
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·      CFCs (engineer bacteria to produce metabolites that break down chlorofluorocarbons—compounds that contribute to the degradation of the ozone layer) <br>
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''Health'' <br>
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·    Stomach ulcers (creating a medication containing engineered bacteria to specifically target and kill Helicobacter pylori – the organism that causes stomach ulcers) <br>
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·      Diabetes (engineered bacteria as Islet cells to produce insulin) <br>
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·      Allergies/Immune system (engineer bacteria to produce antihistamines or alter epitopes) <br>
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·      Bone density (engineer bacteria to produce and secrete calcium and other compounds to help heal broken bones or to prevent osteoporosis) <br>
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''Process Improvement'' <br>
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·      Oil fractionation catalyst  (engineering bacteria to improve the separation of crude oil into valuable fraction and waste fraction)<br>
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·      Nitrate fixation (engineer bacteria to improve nitrogen fixation so not as much fertilizer is needed) <br>
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''Kill Switches'' (engineering bacteria to undergo induced or programmed cell death in order to control the organism) <br>
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==April==
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3/20/2012
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==May==
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Today we attempted to build a circuit and added our project description.
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==June==
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We also tried to select parts to be used in our biological system, we selected a promoter that functions as coliroid and is named BBa_R0082
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3/21/2012
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We worked on selecting the parts we need and selected two mint producing parts we could attempt to use BBa_J45004 and BBa_J45099. The banana producing part is BBa_J45199, an enyzme generator that creates an enzyme to catalyze the process that causes  isoamyl alcohol to break down into isoamyl acetate, which smells like bananas.
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3/23/2012
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Today we hit a magnificent breakthrough in wiki page editing. We can now call ourselves masters. We also planned the system we will create for our project.
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3/26/2012 and 3/27/2012
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Worked on creating a visual of our system. We are working on Tinker Cell to create the whole thing and have a diagram of what we are creating. We know that we will need the light sensor (Cph8) part name BBa_I15010 and the molecule activating our smells will be the OmpRp part name BBa_R0082
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3/28/2012 - 4/3/2012
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Tim did a whole bunch of research and modeling. Austin and Brandon double tasked with "Operation Superman" which was a ginormous success.
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4/4/2012
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Each of the plasmids needed were inserted into compatible bacteria and grown on plates.
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4/5/2012
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Today we moved isolated colonies of each part to broth for growth. We also poured a bunch of plates!
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4/6/2012
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We worked on isolating the plasmids of each part.
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[[Team:BVCAPS_Research_KS | Return to Home]]
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Revision as of 18:23, 18 May 2012

Contents

Notebook

March

Brainstorming

Project ideas:
Pests
· Bacterial Pest attractor (engineer bacteria to produce a substance, such as a pheromone or smell, that attracts and kills pests such as insects)
· Natural pesticides (engineer bacteria to produce a substance that repels or kills pests, such as those that harm crops, that can later be implemented into plants)
Water/Environment:
· Desalination of water (engineering bacteria to get rid of salt in salt water in order to make drinkable water from sea water)
· Getting rid of estrogen mimicking compounds in water (engineering bacteria to degrade them or sequester them)
· Waste treatment (engineering bacteria that can be integrated into waste water treatment)
· CFCs (engineer bacteria to produce metabolites that break down chlorofluorocarbons—compounds that contribute to the degradation of the ozone layer)
Health
· Stomach ulcers (creating a medication containing engineered bacteria to specifically target and kill Helicobacter pylori – the organism that causes stomach ulcers)
· Diabetes (engineered bacteria as Islet cells to produce insulin)
· Allergies/Immune system (engineer bacteria to produce antihistamines or alter epitopes)
· Bone density (engineer bacteria to produce and secrete calcium and other compounds to help heal broken bones or to prevent osteoporosis)
Process Improvement
· Oil fractionation catalyst (engineering bacteria to improve the separation of crude oil into valuable fraction and waste fraction)
· Nitrate fixation (engineer bacteria to improve nitrogen fixation so not as much fertilizer is needed)
Kill Switches (engineering bacteria to undergo induced or programmed cell death in order to control the organism)

April

May

June