Results

Parts created

We created two new parts, [http://partsregistry.org/Part:BBa_K736000 BBa_K736000] (which regulated the transcription of downstream genes) and [http://partsregistry.org/Part:BBa_K736001 BBa_K736001] (which was a TAT signal sequence fused to a red fluorescent protein, E1010). We also assembled two parts, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K736002 BBa_K736002] (which tests the ability of glucose to regulate transcription and uses enhanced RFP as the reporter) and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K736003 BBa_K736003] (which tests for the ability of the TAT sequence to cause secretion of protein outside of the cell and also uses RFP as a reporter). We also created one part that coded for human insulin chain A ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K736004 BBa_K736004]) and one part that coded for human insulin chain B ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K736005 BBa_K736005]).

Name	Type	Description	Length
[http://partsregistry.org/Part:BBa_K736000 BBa_K736000]	Regulatory	Constitutive Promoter (J23100) with MLC (glucose-regulated) binding site	36 bp
[http://partsregistry.org/Part:BBa_K736001 BBa_K736001]	Reporter	TAT signal sequence fused to red fluorescent protein (E1010)	720 bp
[http://partsregistry.org/Part:BBa_K736002 BBa_K736002]	Composite	Constitutive Promoter with MLC-binding-site-regulated enhanced RFP	913 bp
[http://partsregistry.org/Part:BBa_K736003 BBa_K736003]	Composite	pLAC-regulated TAT-RFP construct	948 bp
[http://partsregistry.org/Part:BBa_K736004 BBa_K736004]	Coding	Coding sequence for insulin chain A	72 bp
[http://partsregistry.org/Part:BBa_K736005 BBa_K736005]	Coding	Coding sequence for insulin chain B	99 bp

Proof of Principle

TAT signal sequence

RFP with Cells Media at Different Induction Levels

Glucose Variable Concentrations