Team:CIDEB-UANL Mexico/Wet-lab/Planning

Construction maps description here D:

The work in the laboratory is a fundamental component for iGEM, due that in the realization of projects of Synthetic Biology is required to perform experiments and procedures that allow building genetic innovated pieces, able to generate new functions in the microorganisms which are being manipulated.

Lab Work

Genetic manipulation in iGEM is based in the use of BioBricks, which represents the standardization of Genetic Engineering, allowing that for pasting a piece with another, it can be performed by following a simple and reiterative protocol. In this way, the work in the laboratory could be represented by a flow chart with reiterative methodologies in each desired construction. This techniques collection makes the Genetic Engineering a very simple methodology: Synthetic Biology, which can be followed in a very simple way by junior and high school students.

Circuit

The project for iGEM-CIDEB 2012 includes the construction of a genetic circuit which allows the detection of different concentrations of a determined contaminant compound and, based in these concentrations, shows a reporter which indicates the present levels in the mentioned molecule in the solution. To carry out this idea, it’s required to include a genetic circuit with different modules inside the genetic material of E.coli. Being like this, it is planned to use 13 independent genetic pieces (BioBricks) to build each one of the modules of the complete circuit.