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Team:GPHS Snohomish WA
From 2012hs.igem.org
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Contents |
Arsenic Avengers
- Our team is located at Glacier Peak High School in Snohomish, WA.
- We have eight members.
- Our mentors are Eric Lagally, Ms. Caraballo,and Nick Chavkin (UW bioengineering).
Project Introduction
This semester was focused on introducing ourselves to the competition of iGEM, as well as determining our project. [http://en.wikipedia.org/wiki/Glacier_Peak_High_School The Grizzles] have come to the conclusion to utilize the genes in E. coli that detect and flush out arsenic to determine levels of the element in the waters of Puget Sound. If arsenic is present, we have planned to insert a reporter gene that, when synthesized, will turn the E. coli dark green.
To make this vision a reality, we are using an arsR promoter, a dark green E. chromi reporter from the 2009 Cambridge team's project.
[http://www.consumerreports.org/cro/consumer-reports-magazine-january-2012/arsenic-in-your-juice/index.htm Link to video about Arsenic in Apple Juice]
File:Map.gif
Notebook
6/1-3/12
We attempted to transform E. Coli with test plasmids provided in our kit. Protocol:
1 Make sure set-up is complete
2 Add 2000mL Cacl2 Solution to each tube.
3 "Harvest NEBlO cells: Scrape off the plate for eact tube Resuspend all the tubes
4 Add DNA to each tube 5mL of "RFP Control" to control tube 2-5mL of other plasmids to labeled tubes
5 Incubate on ice for 5 min.
6 Place in water bath for 90 seconds
7 Place tubes on ice for another minute
8 Add 200mL of transformed cells to plate and spread evenly
9 Label 3 plates, with appropiate drug
10 Add 200mL of transformed cells to plate, spread evenly
11Put plates in incubator
Our results were not what we were hoping because the growth of the cells did not occur. The cells may not have taking the plasmids we put in also we suspect this was because of all the refridgerating and unrefridgerating and all the travel the cells had to go through.
This first trial did not have our desired results
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
According to Department of Ecology: State of Washington"Organic and and inorganic arsenic were analyzed in eight fish and shellfish species to determine the appropriateness of placing five Puget Sound waterbodies on the 1998 303(d) list for exceeding EPA human health criteria in edible tissue. The waterbodies in question were Dyes Inlet, Port Washington Narrows, Sinclair Inlet, Port Orchard, and Eagle Harbor. The listings were based on total arsenic data. Results showed the listing criterion of 0.006 ug/g inorganic arsenic was exceeded in all clam samples analyzed. However, this appears to be due to natural conditions in Puget Sound. All crab and fish samples were at or below the listing criterion. It is therefore recommended that these waterbodies be taken off the 303(d) list for arsenic exceedances in edible tissue (12 listings in all)."
Fun!
Our teams favorite snacks consisted of candy, trail mix, and Pirate's Booty. Our team came up with many silly names including, but not limited to, "Dazzle Butters", "Avenging the Ginger", and "Team E.P.I.C". The name "Dazzle Butter" came out of no where and was love instantly. "Avenging the Ginger" is inspired by our redheaded team memeber, who despises this name. "Team E.P.I.C" was supposed to be a acronym for something that related to our team and iGEM, but after about 4 attempts that ended with silly disses toward Cambridge, we gave up and moved on to "Arsenic Avengers", inspired by the new movie- [http://www.imdb.com/title/tt0848228/ The Avengers ]<forum_subtle />
