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Team:GPHS Snohomish WA
From 2012hs.igem.org
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Contents |
Arsenic Avengers
- Our team is located at Glacier Peak High School in Snohomish, WA.
- We have eight members.
- Our mentors are Eric Lagally, Ms. Caraballo,and Nick Chavkin (UW bioengineering).
Project Introduction
This semester was focused on introducing ourselves to the competition of iGEM, as well as determining our project. [http://en.wikipedia.org/wiki/Glacier_Peak_High_School The Grizzles] have come to the conclusion to utilize the genes in E. coli that detect and flush out arsenic to determine levels of the element in the waters of Puget Sound. If arsenic is present, we have planned to insert a reporter gene that, when synthesized, will turn the E. coli dark green.
To make this vision a reality, we are using an arsR promoter, a dark green E. chromi reporter from the 2009 Cambridge team's project.
[http://www.consumerreports.org/cro/consumer-reports-magazine-january-2012/arsenic-in-your-juice/index.htm Link to video about Arsenic in Apple Juice]
File:Map.gif
Notebook
6/1-3/12
We attempted to transform E. Coli with test plasmids provided in our kit. Protocol:
1 Make sure set-up is complete
2 Add 2000mL Cacl2 Solution to each tube.
3 "Harvest NEBlO cells: Scrape off the plate for eact tube Resuspend all the tubes
4 Add DNA to each tube 5mL of "RFP Control" to control tube 2-5mL of other plasmids to labeled tubes
5 Incubate on ice for 5 min.
6 Place in water bath for 90 seconds
7 Place tubes on ice for another minute
8 Add 200mL of transformed cells to plate and spread evenly
9 Label 3 plates, with appropiate drug
10 Add 200mL of transformed cells to plate, spread evenly
11Put plates in incubator
Our results were not what we were hoping because the growth of the cells did not occur. The cells may not have taking the plasmids we put in also we suspect this was because of all the refridgerating and unrefridgerating and all the travel the cells had to go through.
This first trial did not have our desired results
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
Our teams favorite snacks consisted of candy, trail mix, and Pirate's Booty.
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