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Team:Dalton School NY
From 2012hs.igem.org
File:Dalton iGEM.gif 200px | The Dalton School iGEM Team
Our team is composed of 5 juniors in the Advanced Biotechnology and Molecular Biology class at The Dalton School in New York, NY. We are collaborating on the yeast Build-A-Genome project at Johns Hopkins University. | |
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Students in the Advanced Biotechnology and Molecular Biology course at The Dalton School are collaborating with scientists and students at Johns Hopkins University on their effort to construct a synthetic yeast genome. The short term-goals of this project are to construct large libraries of promoters, protein-coding sequences, and terminators that can be easily combined to form functional genes. Dalton students are cloning 30 promoters as part of this effort. In addition, we are also cloning the protein-coding sequences for 6 fluorescent proteins that can eventually be combined with the promoters to test the strength of the promoters. After constructing genes, the individual genes can be assembled into synthetic yeast chromosomes. The long-term goal of the Hopkins Build-A-Genome initiative is to be able to engineer yeast that can be used to solve human problems including combating world hunger, producing alternative sources of fuel, and studying human disease pathways in a simplified system. For example, last year, Hopkins students inserted all of the enzymes necessary to produce vitamin A into yeast. These yeast can be used to bake bread containing vitamin A to supplement the diets of malnourished people. Johns Hopkins iGEM 2011 |  Your team picture | |
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Contents |
Team
Meet our team members!
Project
See the overview of our project here: Please view our lab notebook here: Team:Dalton_School_NY/Project
Notebook
Please view our lab notebook here: Team:Dalton_School_NY/Notebook
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Leslie Mitchell and Patrick Cai in Jef Boeke's lab at Johns Hopkins sent us the sequences of 30 yeast promoters to clone. In addition, we cloned the protein coding sequences of 6 fluorescent proteins that we had received as part of the BioBridge set distributed by Roger Tsien's lab (via Ann Sliski at Princeton University). These are BFP, YFP, GFP, mTangerine, mCherry, and mGrape1.
We designed primer sequences to clone each fragment, cloned them into pUC19, and confirmed their insertion into pUC19 by restriction digest.
Julia B. cloned:
promoters: MET15, GAL1, GAL1-L, GAL1-S, RAD52, RAD50
protein coding sequence: mTangerine
Zoe E. cloned:
promoters: RAD54, RAD55, MRE11, XRS2, REV1, TDH3
protein coding sequence: YFP
Grace F. cloned:
promoters: LIF1, MIG1, TUB1, CSE4, CLN2, CLB2
protein coding sequences: BFP, GFP
Kristen G. cloned:
promoters: ACT1, ENO1, ENO2, TEF2, TEF1, EAF6
protein coding sequence: mCherry
Zoe M. cloned:
promoters: HSP12, HSP26, HSC82, MSN2, MSN4, HHO1
protein coding sequence: mGrape1
Human Practices
What impact does/will your project have on the public?
Sponsors
We are very appreciative of our sponsors!
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