"

From 2012hs.igem.org

Below are some resources that may come in handy as you're working on your projects for iGEM 2012 HS!

Databases and websites to explain concepts:

• Parts Registry Help page is where you can find information about BioBrick Parts and how to use them
• OpenWetWare is a great source for protocols and other lab-related information
• CompuBioTicDB is a database of biological component for synthetic biology

Tutorials:

• Polymerase chain reaction (PCR) (University of Utah)
• Primer design and ordering (OpenWetWare)
• Ligation and plasmid construction (BioBuilder)

Software tools:

TinkerCell is a free "Computer-Aided Design software tool for Synthetic Biology"

GENtle is free software that allows users to edit DNA sequences, generate plasmid maps, and more

Protocols:

• Making competent cells (Registry)
• Competent cell transformation (Registry)
• Ligation (Registry)
• Restriction digest (Registry)
• Transformation lab for students
• Ligation and Transformation module (Bio-Rad)
• Yeast transformation (University of Manitoba)