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Team:BVCAPS Research KS/Notebook
From 2012hs.igem.org
3/20/2012
Today we attempted to build a circuit and added our project description. We also tried to select parts to be used in our biological system, we selected a promoter that functions as coliroid and is named BBa_R0082
3/21/2012
We worked on selecting the parts we need and selected two mint producing parts we could attempt to use BBa_J45004 and BBa_J45099. The banana producing part is BBa_J45199, an enyzme generator that creates an enzyme to catalyze the process that causes isoamyl alcohol to break down into isoamyl acetate, which smells like bananas.
3/23/2012
Today we hit a magnificent breakthrough in wiki page editing. We can now call ourselves masters. We also planned the system we will create for our project.
3/26/2012 and 3/27/2012
Worked on creating a visual of our system. We are working on Tinker Cell to create the whole thing and have a diagram of what we are creating. We know that we will need the light sensor (Cph8) part name BBa_I15010 and the molecule activating our smells will be the OmpRp part name BBa_R0082
3/28/2012 - 4/3/2012
Tim did a whole bunch of research and modeling. Austin and Brandon double tasked with "Operation Superman" which was a ginormous success.
4/4/2012
Each of the plasmids needed were inserted into compatible bacteria and grown on plates.
4/5/2012
Today we moved isolated colonies of each part to broth for growth. We also poured a bunch of plates!
4/6/2012
We worked on isolating the plasmids of each part.
4/9/2012
Today we set down with our instructor to describe the next steps in our project. We also worked on finding published papers of our parts to get a better understanding about them. We also learned that OmpRp is phosphated OmpR and is activated when there is no light. OmpR regulates our system so at night it will be activating the banana smell but we need a activator that will activate the mint during light.
4/10/2012
We set down and worked on modeling our system and came to a realization of what parts we need to add like OmpC and OmpF promoter.
4/11/2012
The systems we are creating were finalized on the Tinkercell program and uploaded to the wiki. We also collaborated with our mentors at Arizona State University. Tim began modeling on Jmol.
