Notebook

From 2012hs.igem.org

3/20/2012

Today we attempted to build a circuit and added our project description. We also tried to select parts to be used in our biological system, we selected a promoter that functions as coliroid and is named BBa_R0082

3/21/2012

We worked on selecting the parts we need and selected two mint producing parts we could attempt to use BBa_J45004 and BBa_J45099. The banana producing part is BBa_J45199, an enyzme generator that creates an enzyme to catalyze the process that causes isoamyl alcohol to break down into isoamyl acetate, which smells like bananas.

3/23/2012

Today we hit a magnificent breakthrough in wiki page editing. We can now call ourselves masters. We also planned the system we will create for our project.

3/26/2012 and 3/27/2012

Worked on creating a visual of our system. We are working on Tinker Cell to create the whole thing and have a diagram of what we are creating. We know that we will need the light sensor (Cph8) part name BBa_I15010 and the molecule activating our smells will be the OmpRp part name BBa_R0082

3/28/2012 - 4/3/2012

Tim did a whole bunch of research and modeling. Austin and Brandon double tasked with "Operation Superman" which was a ginormous success.

4/4/2012

Each of the plasmids needed were inserted into compatible bacteria and grown on plates.

4/5/2012

Today we moved isolated colonies of each part to broth for growth. We also poured a bunch of plates!

4/6/2012

We worked on isolating the plasmids of each part. Return to Home