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Team:Evansville Central
From 2012hs.igem.org
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
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Contents |
Team
The Evansville Central High School iGEM team consists of students in the Project Lead the Way Biomedical Innovations course. Evansville Central is the oldest high school in continuous operation west of the Allegheny Mountains. Evansville, Indiana is located in the southwest corner of the state. The city is positioned on a horseshoe bend of the Ohio River. Central High School is one of five public high schools the Evansville Vanderburgh School Corporation. The high school has 1300 students and is the only high school in Evansville with the complete 4 year Biomedical Science program.
Project
Our project is focusing on the incomplete metabolism of casein and gluten in the human digestive tract. Individuals with dairy intolerance are unable to consume foods containing milk or any milk product that contains casein. Individuals with gluten intolerance are unable to consume foods containing gluten such as wheat/flour products. Our goal is to research the isolation of the dipeptidyl peptidase 4 gene and create a BioBrick which will eventually produce a protein that will aid in the digestion of gluten and casein invitro.
Notebook
2/3/12 - First meeting date Assigned synthetic biology websites and previous iGEM competitors wikis to generate project ideas.
2/10/12 - Brainstormed ideas for our project
2/17/12 - Narrowed our topic human casein and gluten intolerance. Identified a protein of interest to assist in the metabolism of casein and gluten
2/24/12 - Research assignments: a. Normal Gluten metabolism b. Normal Casein metabolism c. Molecular structure of gluten and casein d. Mechanism pathway for DPP-4 (Dipeptidyl peptidase-4) e. Analogs for DPP-4 f. Effects of poor metabolism of gluten and casein Assigned articles for research
3/6/12 - Introduced the Mendely citation manager
3/16/12 - Created a shared Google document for all team members to access and share information from their research assignments from 2/24/12
3/23/12 - Ice cream party
4/4/12 - Figured out that DPP-4 is naturally produced in humans and in patients with Celiac disease DPP-4 maybe deficient. We are going to target DPP-4 for isolation from human cells.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot? Our favorite team snack is Russian sweet bread that is filled with fruit. Its called Peroshki. We also like to drink Mountain Dew and eat chips.
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