Team:CIDEB-UANL Mexico/Wet-lab/Planning

To work in the laboratory we made a plan about how to build the circuit. The principal processes are cut and ligate. To cut the biobricks we need restriction enzymes type II. These enzymes cut in a specific place. Here we hace an example about how to cut and ligate the pieces 1-6M and 3-12M:

The part 1-6M is cut with the EcoRI and SpeI enzymes