Team:Heidelberg LSL/Parts

From 2012hs.igem.org

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iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg


Note: all parts were carefully characterized before submission to the registry. Details about the characterization can be found in our Measurement Section or directly on the different parts pages in the registry.

NameDescriptionRegistry linkPart typeAvailability
precA_LacZThis is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).

BBa_K862000

Measurementpartsregistry
psulA_LacZThis is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). BBa_K862001 Measurement partsregistry
precB_LacZThis is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).BBa_K862002 Measurement partsregistry
precBThe recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.

RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA
CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3'

RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC
GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'
BBa_K862003 Regulatory can be cloned on oligo basis (sequence provided)