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From 2012hs.igem.org

Advisors

Jef Boeke and colleagues at Johns Hopkins University School of Medicine, including postdocs Leslie Mitchell and Patrick Cai, provided the design and inspiration for this project.

Jennifer Hackett (our teacher and former Johns Hopkins grad student) advised students as they worked on this project.

Attributions

Leslie Mitchell and Patrick Cai in Jef Boeke's lab at Johns Hopkins sent us the sequences of 30 yeast promoters to clone. In addition, we cloned the protein coding sequences of 6 fluorescent proteins that we had received as part of the BioBridge set distributed by Roger Tsien's lab (via Ann Sliski at Princeton University). These are BFP, YFP, GFP, mTangerine, mCherry, and mGrape1.

We designed primer sequences to clone each fragment, cloned them into pUC19, and confirmed their insertion into pUC19 by restriction digest.

Julia B. cloned:

promoters: MET15, GAL1, GAL1-L, GAL1-S, RAD52, RAD50

protein coding sequence: mTangerine

Zoe E. cloned:

promoters: RAD54, RAD55, MRE11, XRS2, REV1, TDH3

protein coding sequence: YFP

Grace F. cloned:

promoters: LIF1, MIG1, TUB1, CSE4, CLN2, CLB2

protein coding sequences: BFP, GFP

Kristen G. cloned:

promoters: ACT1, ENO1, ENO2, TEF2, TEF1, EAF6

protein coding sequence: mCherry

Zoe M. cloned:

promoters: HSP12, HSP26, HSC82, MSN2, MSN4, HHO1

protein coding sequence: mGrape1