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From 2012hs.igem.org

Our team is consisted of four students who are fond of thinking creatively, sharing our knowledge with others and making contributions to the society. We hope that iGEM 2012 could be a great opportunity for us to get our feet wet in the field of synthetic biology and interact with many other students around the world who are also interested in this field!	File:CSIA SouthKorea logo.png 200px
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team CSIA_SouthKorea

Official Team Profile

Contents 1 Team 2 Brainstorming 2.1 Ideas from imagination 2.1.1 Lactose intolerance curing E. coli 2.2 Ideas based on previous iGEM teams 3 Project 4 Diary 5 Results/Conclusions 6 Safety 7 Attributions 8 Human Practices 9 Fun!

Team

Brainstorming

Ideas from imagination

Lactose intolerance curing E. coli

We thought about the mechanism of making E. Coli to do apoptosis when there is excessive nutrient in human colon (e.g. when a lactose intolerant individual intakes dairy product) so that it prevents excessive growth of E. Coli culture and reduces lactose-intolerant symptoms, such as diarrhea.

• Mechanism step (hypothetical) 1. Sensing lactose

We could not find an adaptable lactose-sensing gene. Therefore, it would be better to adopt the glucose sensing mechanism of Baker's yeast.

1. Put in lactase making gene inside the E. Coli's DNA. Put in the spliced mRNA version (of course it would be transferred into DNA by using reverse transcriptase before putting the mRNA strand into the E. Coli's gene) of Baker's yeast's glucose-sensing gene.
2. When there is lactase inside human colon, the lactase-producing gene will express and E.Coli will produce lactase.
3. Lactase will decompose lactose into galactose and glucose.
4. The glucose sensing gene will detect glucose.

• Mechanism step 2. Apoptosis signal

In ‘Baker's yeast’, the gene HXT is expressed when glucose is detected. Instead of HXT, if we put the gene that induces cell death in parts registry, we predicted that the cell will apoptosis when the glucose is detected.

• Things to consider :

Since the cell should not die from the signal it sent to itself, the genetically modified E.Coli should be resistant to the apoptosis signal sent from itself. Further, the power of the signal should be controlled in its time and magnitude.

Ideas based on previous iGEM teams

Project

Diary

• 2012.02.16: meeting with professor
• Our team had a meeting with professor In-Geol Choi in order to discuss our topic for the igem project. We had some creative ideas of our own, but we weren’t sure if they were appropriate topics for this project. As our discussion went on, professor Choi gave us some advices in selecting the topic:
• First, the project should have some kind of purpose – something like ‘saving environment’. We need to synthesize and create biological machine of certain function that can fulfill the fundamental purpose of our own.
• Second, considering that we don’t have much time nor the professional knowledge for the research and experiments, as high school students, it might be more appropriate to work with relatively easy project. He recommended us to do some research on websites such as biobuilders, and read about some labs that students can conduct.
• Third, note the parts registry that we can use. In parts registry site ( http://partsregistry.org/Catalog) we can find out which devices and functions we are able to use, and that will help us with selecting our topic. We decided to consider these points and brainstorm more about the topics. We are to determine our topic by the end of this February.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

Attributions

Human Practices

Fun!

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