Team:Heidelberg LSL/Notebook methods

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iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Methods

Transformation

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Inoculation of LB agar plates

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Inoculation of liquid LB medium

Qiagen Miniprep

Restriction digest

SAP digest

Gel electrophoresis

Gel extraction

Qiagen nucleotide removal kit

Ligation digest

Oligo annealing

Colony PCR

Sequencing

X-Gal assay

ONPG assay

Use of the photometer

Use of the plate reader

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