Team:Heidelberg LSL/Parts

From 2012hs.igem.org

(Difference between revisions)

Revision as of 03:01, 17 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Note: All parts were carefully characterized before submission to the registry. Details about the characterization can be found in our Measurement Section or directly on the different parts pages in the registry.

Name	Description	Registry link	Part type	Availability
precA_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).	BBa_K862000	Measurement	partsregistry
psulA_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).	BBa_K862001	Measurement	partsregistry
precB_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).	BBa_K862002	Measurement	partsregistry
precB	The RecB promoter sequence was obtained from the E.coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part. RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'	BBa_K862003	Regulatory	can be cloned on oligo basis (sequence provided)

@@ Line 30: / Line 30: @@
 <td>precA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/c/c0/BBa_K862000.PNG"/><br/>
 <br/>
-This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part <a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (part <a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td>
+This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (part <a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (part <a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td>
 <p>
@@ Line 40: / Line 40: @@
 <td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/>
 <br/>
-This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part <a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (part <a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
+This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (part <a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (part <a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
 </td><td>partsregistry</td></tr>
@@ Line 46: / Line 46: @@
 <td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/>
 <br/>
-This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part <a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (part <a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
+This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (part <a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (part <a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
 </td><td>partsregistry</td></tr>
@@ Line 54: / Line 54: @@
 <td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
 <br/>
-The recB promoter sequence was taken for the <i>E. coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
+The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
-We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.
+We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.
 <br>
 <br/>RecB_fw:	5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br>