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Team:CIDEB-UANL Mexico/Project/Abstract
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| - | <a name=" | + | <a name="Project"></a>Overview |
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| - | <p>Nowadays due to the contamination caused by pollutants such as heavy metals, it is important the implementation of simple and complete detection methods. This project aims to create a biosensor that may detect the presence of heavy metals in water. In order to construct the biosensor, a genetic circuit was built into E. coli. The circuit represents a model, which works with Arabinose. It is a semi-quantitative biosensor. The circuit has 3 parts: High concentration, Low concentration and Stand-by state. Each part of the circuit has a different response: when there is a high concentration of Arabinose the bacteria shows a yellow fluorescent color; when the concentration is low it uses a sensitivity tunner that increases the response from the same amount of Arabinose, which makes the bacteria to appear in a red fluorescence; and when there is no Arabinose in the sample, the bacteria shows a green fluorescence | + | <p>Nowadays due to the contamination caused by pollutants such as heavy metals, it is important the implementation of simple and complete detection methods. This project aims to create a biosensor that may detect the presence of heavy metals in water. In order to construct the biosensor, a genetic circuit was built into E. coli. The circuit represents a model, which works with Arabinose. It is a semi-quantitative biosensor. The circuit has 3 parts: High concentration, Low concentration and Stand-by state. Each part of the circuit has a different response: when there is a high concentration of Arabinose the bacteria shows a yellow fluorescent color; when the concentration is low it uses a sensitivity tunner that increases the response from the same amount of Arabinose, which makes the bacteria to appear in a red fluorescence; and when there is no Arabinose in the sample, the bacteria shows a green fluorescence. </p> |
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| - | <div class="lateral-button"><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Modelling/ | + | <div class="lateral-button"><a href="https://2011.igem.org/Team:UANL_Mty-Mexico/Modelling/Proejct#Abstract">Abstract</a></div> |
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Revision as of 21:35, 16 June 2012
Nowadays due to the contamination caused by pollutants such as heavy metals, it is important the implementation of simple and complete detection methods. This project aims to create a biosensor that may detect the presence of heavy metals in water. In order to construct the biosensor, a genetic circuit was built into E. coli. The circuit represents a model, which works with Arabinose. It is a semi-quantitative biosensor. The circuit has 3 parts: High concentration, Low concentration and Stand-by state. Each part of the circuit has a different response: when there is a high concentration of Arabinose the bacteria shows a yellow fluorescent color; when the concentration is low it uses a sensitivity tunner that increases the response from the same amount of Arabinose, which makes the bacteria to appear in a red fluorescence; and when there is no Arabinose in the sample, the bacteria shows a green fluorescence.
