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Team:Heidelberg LSL/Project UVsensors
From 2012hs.igem.org
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| - | + | <p>The intention was to generate constructs that are able to sense irradiation and respond to UV light with a colorful output. </p> | |
| - | <p> | + | <br> |
| + | <p>First, we looked for proper <i>E. coli</i> promoters known to be involved in the SOS response. While we found RecA in the <a href="http://partsregistry.org/Main_Page">registry</a>, RecB, RecC and SulA promoters were generated custom-made oligonucleotides. Sequences were taken from the <a href="http://ecoliwiki.net/Main_Page">EcoliWiki</a></p> | ||
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<div id="news"> | <div id="news"> | ||
Revision as of 20:07, 16 June 2012
Strategy
Overview
SOS response promoters from E. coli were either annealed from synthetic oligos or taken from the registry. Promoters were ligated together with digested standard reporter constructs into standard backbone thereby generating our final radiation sensor construct.
Description
The intention was to generate constructs that are able to sense irradiation and respond to UV light with a colorful output.
First, we looked for proper E. coli promoters known to be involved in the SOS response. While we found RecA in the registry, RecB, RecC and SulA promoters were generated custom-made oligonucleotides. Sequences were taken from the EcoliWiki
