Team:SouthBendMishawakaIN

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Revision as of 19:41, 12 June 2012

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Millions of people worldwide are exposed to toxic levels of arsenic through contaminated

drinking water. In last year’s project, we began the development of an E. coli-based

arsenic biosensor using an arsenic-induced promoter from the Registry (K190015). Like the

Groningen Team, we found that this promoter is “leaky,” having a constant low level of

activity. We explored ways of reducing background activity to improve the sensitivity of

our device. We have built and characterized biosensors using K190015 and a number of

chemiluminescence generators from Vibrio fisheri and two species of fireflies. In order to

standardize our biosensor we have designed and built a simple luminometer. Paired with our

device, this can be used to inexpensively and rapidly detect arsenic in water, even by

people in underdeveloped parts of the world.

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 !align="center"|[[Team:SouthBend-Mishawaka-HS/Attributions|<font color=#99FFFF >Sponsors</font>]]
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+<center>
+<h4>Abstract</h4>
+<Br>
+Millions of people worldwide are exposed to toxic levels of arsenic through contaminated
+drinking water.  In last year’s project, we began the development of an E. coli-based
+arsenic biosensor using an arsenic-induced promoter from the Registry (K190015).  Like the
+Groningen Team, we found that this promoter is “leaky,” having a constant low level of
+activity.  We explored ways of reducing background activity to improve the sensitivity of
+our device.  We have built and characterized biosensors using K190015 and a number of
+chemiluminescence generators from Vibrio fisheri and two species of fireflies.  In order to
+standardize our biosensor we have designed and built a simple luminometer.   Paired with our
+device, this can be used to inexpensively and rapidly detect arsenic in water, even by
+people in underdeveloped parts of the world.
+</center>