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Team:Heidelberg LSL/Notebook methods
From 2012hs.igem.org
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Revision as of 17:59, 11 June 2012
Methods
Transformation
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Inoculation of LB agar plates
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Inoculation of liquid LB medium
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Qiagen Miniprep
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Restriction digest
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SAP digest
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Gel electrophoresis
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Gel extraction
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Qiagen nucleotide removal kit
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Ligation digest
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Oligo annealing
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Colony PCR
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Sequencing
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X-Gal assay
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ONPG assay
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Use of the photometer
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Use of the plate reader
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