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Team:Dalton School NY/Team
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| - | + | protein coding sequence: mGrape1 | |
Revision as of 18:16, 29 May 2012
Advisors
Jef Boeke and colleagues at Johns Hopkins University School of Medicine, including postdocs Leslie Mitchell and Patrick Cai, provided the design and inspiration for this project.
Jennifer Hackett (our teacher and former Johns Hopkins grad student) advised students as they worked on this project.
Attributions
Leslie Mitchell and Patrick Cai in Jef Boeke's lab at Johns Hopkins sent us the sequences of 30 yeast promoters to clone. In addition, we cloned the protein coding sequences of 6 fluorescent proteins that we had received as part of the BioBridge set distributed by Roger Tsien's lab (via Ann Sliski at Princeton University). These are BFP, YFP, GFP, mTangerine, mCherry, and mGrape1.
We designed primer sequences to clone each fragment, cloned them into pUC19, and confirmed their insertion into pUC19 by restriction digest.
Julia B. cloned:
promoters: MET15, GAL1, GAL1-L, GAL1-S, RAD52, RAD50
protein coding sequence: mTangerine
Zoe E. cloned:
promoters: RAD54, RAD55, MRE11, XRS2, REV1, TDH3
protein coding sequence: YFP
Grace F. cloned:
promoters: LIF1, MIG1, TUB1, CSE4, CLN2, CLB2
protein coding sequences: BFP, GFP
Kristen G. cloned:
promoters: ACT1, ENO1, ENO2, TEF2, TEF1, EAF6
protein coding sequence: mCherry
Zoe M. cloned:
promoters: HSP12, HSP26, HSC82, MSN2, MSN4, HHO1
protein coding sequence: mGrape1
