Team:Heidelberg LSL/Parts

From 2012hs.igem.org

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Revision as of 21:20, 16 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Note: All parts were carefully characterized before submission to the registry. Details about the characterization can be found in our Measurement Section or directly on the different parts pages in the registry.

Name	Description	Registry link	Part type	Availability
precA_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).	BBa_K862000	Measurement	partsregistry
psulA_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).	BBa_K862001	Measurement	partsregistry
precB_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).	BBa_K862002	Measurement	partsregistry
precB	The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part. RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'	BBa_K862003	Regulatory	can be cloned on oligo basis (sequence provided)

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-<td>precB<br/><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/></td><td>The recB promoter sequence was taken for the <i>E. coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
+<td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
+The recB promoter sequence was taken for the <i>E. coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
 We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.
 <br>