Team:WarrenCentral WCC IN

From 2012hs.igem.org

(Difference between revisions)
(Detection of Mercury Using Yeast)
m
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
-
 
-
<html>
 
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #fe2b33; /*background-color: #fe2b33;*/">
 
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; /*color: #f6f6f6*/; padding: 5px;">
 
-
This is a template page. READ THESE INSTRUCTIONS.
 
-
</div>
 
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
 
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
-
</div>
 
-
<div id="warning" style="/*text-align: center;*/ font-weight: bold; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
 
-
You <strong>MUST</strong> have the following information on your wiki:
 
-
<ul style="font-weight:normal;">
 
-
<li>a team description</li>
 
-
<li>project description</li>
 
-
<li>safety information (did your team take a safety training course? were you supervised in the lab?)</li>
 
-
<li>team attribution (who did what part of your project?)</li>
 
-
</ul>
 
-
You may also wish to add other page such as:
 
-
<ul style="font-weight:normal;">
 
-
<li>lab notebook</li>
 
-
<li>sponsor information</li>
 
-
<li>other information</li>
 
-
</ul>
 
-
REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2012hs.igem.org/Team:WarrenCentral_WCC_IN/Our_Pets</span>
 
-
</div>
 
-
</div>
 
-
</html>
 
-
 
-
<!-- *********************** End of the alert box *********************** -->
 
-
 
-
 
{|align="justify"
{|align="justify"

Revision as of 17:37, 21 March 2012

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. File:WarrenCentral WCC IN logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Your team picture
Team WarrenCentral_WCC_IN


Official Team Profile

Contents

Team

Tell us about your team, your school!


Project

Exposure to mercury is a widespread problem that affects many people all over the world. Most people ingest mercury through water sources. Mercury in water can arise from runoff from farms, chemical and industrial plants, household products in the trash, and sewage. Three types of mercury can adversely affect humans. Elemental, inorganic, and methyl mercury can all harm humans if ingested. Inorganic mercury is the most common form in drinking water and can cause kidney damage if enough is taken in. Methyl mercury is found in fish and humans can be exposed if they eat too much mercury-containing fish. Mercury ingestion can cause both acute and chronic symptoms.

We are using Saccharomyces cerevisiae yeast as a tool to detect mercury. In yeast, there are a number of transcription factors and genes that respond to oxidative stress and toxic metals. The yes associated protein (YAP) family is a family of transcription factors that is involved with oxidative stress regulation and redox homeostasis. They affect a number of genes, but we are focusing on GSH1 and GSH2. These genes are involved in the glutathione pathway. Glutathione is an antioxidant that protects the cell from oxidative stress.

In order to detect mercury, we are using several biological parts included in the BioBrick. The Kozak + mCherry translational unit is being used to give off a red fluorescent glow when the mercury is detected. In the plasmid, we will include the GSH2 promoter and the ADH1 terminator.

Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


<forum_subtle />

Retrieved from "http://2012hs.igem.org/Team:WarrenCentral_WCC_IN"