Team:SouthBendMishawakaIN

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DNA Arcitects
DNA Arcitects
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SouthBendMishawakaIN
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SouthBend MishawakaIN
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Team: SouthBendMishawakaIN
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Title: The "Arsenator:" Design and development of a biosensor for arsenic in ground water
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<h4>Abstract</h4>
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Millions of people worldwide are exposed to toxic levels of arsenic through
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contaminated
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Millions of people worldwide are exposed to toxic levels of arsenic through contaminated  
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drinking water. In last year’s project, we began the development of an E. coli-based  
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drinking water. In last year’s project, we began the development of an E. coli-based
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arsenic biosensor using an arsenic-induced promoter from the Registry (K190015). Like the  
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arsenic biosensor using an arsenic-induced promoter from the Registry (K190015). Like the
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Groningen Team, we found that this promoter is “leaky,” having a constant low level of  
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Groningen Team, we found that this promoter is “leaky,” having a constant low level of
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activity. We explored ways of reducing background activity to improve the sensitivity of  
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activity. We explored ways of reducing background activity to improve the sensitivity of
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our device. We have built and characterized biosensors using K190015 and a number of  
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our device. We have built and characterized biosensors using K190015 and a number of
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chemiluminescence generators from Vibrio fisheri and two species of fireflies. In order to  
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chemiluminescence generators from Vibrio fisheri and two species of fireflies. In order to
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standardize our biosensor we have designed and built a simple luminometer.   Paired with our  
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standardize our biosensor we have designed and built a simple luminometer. Paired with our
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device, this can be used to inexpensively and rapidly detect arsenic in water, even by  
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device, this can be used to inexpensively and rapidly detect arsenic in water, even by
people in underdeveloped parts of the world.
people in underdeveloped parts of the world.
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Revision as of 22:22, 14 June 2012

DNA Arcitects
SouthBend MishawakaIN


Home
Project
Notebook
Safety
Model
Parts
Sponcers

Abstract


Millions of people worldwide are exposed to toxic levels of arsenic through contaminated drinking water. In last year’s project, we began the development of an E. coli-based arsenic biosensor using an arsenic-induced promoter from the Registry (K190015). Like the Groningen Team, we found that this promoter is “leaky,” having a constant low level of activity. We explored ways of reducing background activity to improve the sensitivity of our device. We have built and characterized biosensors using K190015 and a number of chemiluminescence generators from Vibrio fisheri and two species of fireflies. In order to standardize our biosensor we have designed and built a simple luminometer. Paired with our device, this can be used to inexpensively and rapidly detect arsenic in water, even by people in underdeveloped parts of the world.

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