Team:Dalton School NY

From 2012hs.igem.org

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|'''The Dalton School iGEM Team:
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|'''The Dalton School iGEM Team'''  
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Construction of a Portion of a Parts Library for the Johns Hopkins Yeast Build-A-Genome Project
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'''  
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Our team is composed of 5 juniors in the Advanced Biotechnology and Molecular Biology class at The Dalton School in New York, NY. We are collaborating on the yeast Build-A-Genome project at Johns Hopkins University.
Our team is composed of 5 juniors in the Advanced Biotechnology and Molecular Biology class at The Dalton School in New York, NY. We are collaborating on the yeast Build-A-Genome project at Johns Hopkins University.
|[[Image:Dalton_School_NY_logo.png|200px|right|frame]]
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===Results/Conclusions===
===Results/Conclusions===
What did you achieve over the course of your semester?
What did you achieve over the course of your semester?
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===Safety===
===Safety===
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===Attributions===
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Leslie Mitchell and Patrick Cai in Jef Boeke's lab at Johns Hopkins sent us the sequences of 30 yeast promoters to clone. In addition, we cloned the protein coding sequences of 6 fluorescent proteins that we had received as part of the BioBridge set distributed by Roger Tsien's lab (via Ann Sliski at Princeton University). These are BFP, YFP, GFP, mTangerine, mCherry, and mGrape1.
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We designed primer sequences to clone each fragment, cloned them into pUC19, and confirmed their insertion into pUC19 by restriction digest.
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Julia B. cloned:
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    promoters: MET15, GAL1, GAL1-L, GAL1-S, RAD52, RAD50
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    protein coding sequence: mTangerine
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Zoe E. cloned:
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    promoters: RAD54, RAD55, MRE11, XRS2, REV1, TDH3
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    protein coding sequence: YFP
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Grace F. cloned:
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    promoters: LIF1, MIG1, TUB1, CSE4, CLN2, CLB2
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    protein coding sequences: BFP, GFP
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Kristen G. cloned:
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    promoters: ACT1, ENO1, ENO2, TEF2, TEF1, EAF6
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    protein coding sequence: mCherry
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Zoe M. cloned:
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    promoters: HSP12, HSP26, HSC82, MSN2, MSN4, HHO1
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    protein coding sequence: mGrape1
===Human Practices===
===Human Practices===

Revision as of 18:09, 29 May 2012

File:Dalton iGEM.gif
200px
The Dalton School iGEM Team

Our team is composed of 5 juniors in the Advanced Biotechnology and Molecular Biology class at The Dalton School in New York, NY. We are collaborating on the yeast Build-A-Genome project at Johns Hopkins University.

File:Dalton School NY logo.png
200px

Students in the Advanced Biotechnology and Molecular Biology course at The Dalton School are collaborating with scientists and students at Johns Hopkins University on their effort to construct a synthetic yeast genome. The short term-goals of this project are to construct large libraries of promoters, protein-coding sequences, and terminators that can be easily combined to form functional genes. Dalton students are cloning 30 promoters as part of this effort. In addition, we are also cloning the protein-coding sequences for 6 fluorescent proteins that can eventually be combined with the promoters to test the strength of the promoters. After constructing genes, the individual genes can be assembled into synthetic yeast chromosomes. The long-term goal of the Hopkins Build-A-Genome initiative is to be able to engineer yeast that can be used to solve human problems including combating world hunger, producing alternative sources of fuel, and studying human disease pathways in a simplified system. For example, last year, Hopkins students inserted all of the enzymes necessary to produce vitamin A into yeast. These yeast can be used to bake bread containing vitamin A to supplement the diets of malnourished people. Johns Hopkins iGEM 2011

Your team picture
Team Dalton_School_NY


Official Team Profile

Contents

Team

Project

See the overview of our project here: Please view our lab notebook here: Team:Dalton_School_NY/Project

Notebook

Please view our lab notebook here: Team:Dalton_School_NY/Notebook

Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Leslie Mitchell and Patrick Cai in Jef Boeke's lab at Johns Hopkins sent us the sequences of 30 yeast promoters to clone. In addition, we cloned the protein coding sequences of 6 fluorescent proteins that we had received as part of the BioBridge set distributed by Roger Tsien's lab (via Ann Sliski at Princeton University). These are BFP, YFP, GFP, mTangerine, mCherry, and mGrape1.

We designed primer sequences to clone each fragment, cloned them into pUC19, and confirmed their insertion into pUC19 by restriction digest.


Julia B. cloned: 

    promoters: MET15, GAL1, GAL1-L, GAL1-S, RAD52, RAD50

    protein coding sequence: mTangerine

Zoe E. cloned: 

    promoters: RAD54, RAD55, MRE11, XRS2, REV1, TDH3

    protein coding sequence: YFP

Grace F. cloned: 

    promoters: LIF1, MIG1, TUB1, CSE4, CLN2, CLB2

    protein coding sequences: BFP, GFP

Kristen G. cloned: 

    promoters: ACT1, ENO1, ENO2, TEF2, TEF1, EAF6

    protein coding sequence: mCherry

Zoe M. cloned: 

    promoters: HSP12, HSP26, HSC82, MSN2, MSN4, HHO1

    protein coding sequence: mGrape1

Human Practices

What impact does/will your project have on the public?


Sponsors

We are very appreciative of our sponsors!

File:Dalton sponsors.jpg

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