"
Team:Heidelberg LSL/Notebook-23/02/2012
From 2012hs.igem.org
(Difference between revisions)
Sholderbach (Talk | contribs) |
|||
| Line 18: | Line 18: | ||
<h2> Biosensor Construction - 23/02/2012 </h2> | <h2> Biosensor Construction - 23/02/2012 </h2> | ||
<!--Bitte ab hier den Inhalt hineinschreiben--> | <!--Bitte ab hier den Inhalt hineinschreiben--> | ||
| - | <b>Transformation of GFP (BBa_ E0240) and RecA (BBa_J22106) from registry 2010 spring distribution into E. coli Top10 cells</b><br /><br /> | + | *<b>Transformation of GFP (BBa_ E0240) and RecA (BBa_J22106) from registry 2010 spring distribution into E. coli Top10 cells</b><br /><br /> |
<b>Protocol:</b><br /> | <b>Protocol:</b><br /> | ||
10 ul water is added to the corresponding well, DNA is solved by incubation for 15 min at room temperature. 5 ul of DNA solution are added to 50 ul of chemocompetent Top10 cells and incubated on ice for 20 min. Afterwards, a heat shock is performed by incubation the bacteria on 42°C/50 s. After another 2 min on ice, the bacteria are plated onto LB-Amp agar plates. | 10 ul water is added to the corresponding well, DNA is solved by incubation for 15 min at room temperature. 5 ul of DNA solution are added to 50 ul of chemocompetent Top10 cells and incubated on ice for 20 min. Afterwards, a heat shock is performed by incubation the bacteria on 42°C/50 s. After another 2 min on ice, the bacteria are plated onto LB-Amp agar plates. | ||
Latest revision as of 19:06, 6 June 2012
Notebook
Welcome to our notebook!
Here you will find the documentation of our laboratory work of the last few month in diary form. This notebook comprises the work in three phases:
|
|
|
Biosensor Construction - 23/02/2012
- Transformation of GFP (BBa_ E0240) and RecA (BBa_J22106) from registry 2010 spring distribution into E. coli Top10 cells
Protocol:
10 ul water is added to the corresponding well, DNA is solved by incubation for 15 min at room temperature. 5 ul of DNA solution are added to 50 ul of chemocompetent Top10 cells and incubated on ice for 20 min. Afterwards, a heat shock is performed by incubation the bacteria on 42°C/50 s. After another 2 min on ice, the bacteria are plated onto LB-Amp agar plates.
