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Team:CSIA SouthKorea
From 2012hs.igem.org
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=Brainstorming= | =Brainstorming= | ||
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==Ideas from imagination== | ==Ideas from imagination== | ||
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| + | ===Lactose intolerance curing E. coli=== | ||
| + | We thought about the mechanism of making E. Coli to do apoptosis when there is excessive nutrient in human colon (e.g. when a lactose intolerant individual intakes dairy product) so that it prevents excessive growth of E. Coli culture and reduces lactose-intolerant symptoms, such as diarrhea. | ||
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| + | * Mechanism step (hypothetical) 1. Sensing lactose | ||
| + | : We could not find an adaptable lactose-sensing gene. Therefore, it would be better to adopt the glucose sensing mechanism of Baker's yeast. | ||
| + | 1) Put in lactase making gene inside the E. Coli's DNA. Put in the spliced mRNA version (of course it would be transferred into DNA by using reverse transcriptase before putting the mRNA strand into the E. Coli's gene) of Baker's yeast's glucose-sensing gene. | ||
| + | 2) When there is lactase inside human colon, the lactase-producing gene will express and E.Coli will produce lactase. | ||
| + | 3) Lactase will decompose lactose into galactose and glucose. | ||
| + | 4) The glucose sensing gene will detect glucose. | ||
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==Ideas based on previous iGEM teams== | ==Ideas based on previous iGEM teams== | ||
Revision as of 05:19, 3 March 2012
| Our team is consisted of four students who are fond of thinking creatively, sharing our knowledge with others and making contributions to the society. We hope that iGEM 2012 could be a great opportunity for us to get our feet wet in the field of synthetic biology and interact with many other students around the world who are also interested in this field! | |
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) |  Your team picture |
| Team CSIA_SouthKorea |
| Official Team Profile |
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Contents |
Team
Brainstorming
Ideas from imagination
Lactose intolerance curing E. coli
We thought about the mechanism of making E. Coli to do apoptosis when there is excessive nutrient in human colon (e.g. when a lactose intolerant individual intakes dairy product) so that it prevents excessive growth of E. Coli culture and reduces lactose-intolerant symptoms, such as diarrhea.
- Mechanism step (hypothetical) 1. Sensing lactose
- We could not find an adaptable lactose-sensing gene. Therefore, it would be better to adopt the glucose sensing mechanism of Baker's yeast.
1) Put in lactase making gene inside the E. Coli's DNA. Put in the spliced mRNA version (of course it would be transferred into DNA by using reverse transcriptase before putting the mRNA strand into the E. Coli's gene) of Baker's yeast's glucose-sensing gene. 2) When there is lactase inside human colon, the lactase-producing gene will express and E.Coli will produce lactase. 3) Lactase will decompose lactose into galactose and glucose. 4) The glucose sensing gene will detect glucose.
Ideas based on previous iGEM teams
Project
Diary
- 2012.02.16: meeting with professor
- Our team had a meeting with professor In-Geol Choi in order to discuss our topic for the igem project. We had some creative ideas of our own, but we weren’t sure if they were appropriate topics for this project. As our discussion went on, professor Choi gave us some advices in selecting the topic:
- First, the project should have some kind of purpose – something like ‘saving environment’. We need to synthesize and create biological machine of certain function that can fulfill the fundamental purpose of our own.
- Second, considering that we don’t have much time nor the professional knowledge for the research and experiments, as high school students, it might be more appropriate to work with relatively easy project. He recommended us to do some research on websites such as biobuilders, and read about some labs that students can conduct.
- Third, note the parts registry that we can use. In parts registry site ( http://partsregistry.org/Catalog) we can find out which devices and functions we are able to use, and that will help us with selecting our topic. We decided to consider these points and brainstorm more about the topics. We are to determine our topic by the end of this February.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
Attributions
Human Practices
Fun!
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