Team:Evansville Central

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Revision as of 18:50, 8 June 2012

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have the following information on your wiki:

a team description
project description
safety information (did your team take a safety training course? were you supervised in the lab?)
team attribution (who did what part of your project?)

You may also wish to add other page such as:

lab notebook
sponsor information
other information

REMEMBER, keep all of your pages within your teams namespace.
Example: 2012hs.igem.org/Team:Evansville_Central/Our_Pets

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	File:Evansville Central team.png Your team picture
	Team Evansville_Central

Official Team Profile

The Evansville Central High School iGEM team consists of students in the Project Lead the Way Biomedical Innovations course. Evansville Central is the oldest high school in continuous operation west of the Allegheny Mountains. Evansville, Indiana is located in the southwest corner of the state. The city is positioned on a horseshoe bend of the Ohio River. Central High School is one of five public high schools the Evansville Vanderburgh School Corporation. The high school has 1300 students and is the only high school in Evansville with the complete 4 year Biomedical Science program.

Project

Our project is focusing on the incomplete metabolism of casein and gluten in the human digestive tract. Individuals with dairy intolerance are unable to consume foods containing milk or any milk product that contains casein. Individuals with gluten intolerance are unable to consume foods containing gluten such as wheat/flour products. Our goal is to research the isolation of the dipeptidyl peptidase 4 gene and create a BioBrick which will eventually produce a protein that will aid in the digestion of gluten and casein invitro.

Notebook

2/3/12 - First meeting date Assigned synthetic biology websites and previous iGEM competitors wikis to generate project ideas.

2/10/12 - Brainstormed ideas for our project

2/17/12 - Narrowed our topic human casein and gluten intolerance. Identified a protein of interest to assist in the metabolism of casein and gluten

2/24/12 - Research assignments: a. Normal Gluten metabolism b. Normal Casein metabolism c. Molecular structure of gluten and casein d. Mechanism pathway for DPP-4 (Dipeptidyl peptidase-4) e. Analogs for DPP-4 f. Effects of poor metabolism of gluten and casein Assigned articles for research

3/6/12 - Introduced the Mendely citation manager

3/16/12 - Created a shared Google document for all team members to access and share information from their research assignments from 2/24/12

3/23/12 - Ice cream party

4/4/12 - Figured out that DPP-4 is naturally produced in humans and in patients with Celiac disease DPP-4 maybe deficient. We are going to target DPP-4 for isolation from human cells.

4/18/12 - Trouble finding genomic sequences for DPP-4 and could only find whole genome shotgun sequences.

4/27/12 - Donut party. Team discussed other options for proteins in bacterial organisms that would breakdown casein and gluten. Team discussed the University of Washington's project in 2011 on gluten intolerance.

5/11/12 - Using NCBI genbank database in conjunction with the ensembl database we were able to figure out that DPP-4 genomic DNA was spread out over 60,000 base pairs with many large intervening regions between the coding sequences. Our team decided what our opinions were for continuing DPP-4 as our Bio-Brick. We determined the best course of actions was to amplify a 3000-4000 base pair region for DPP-4. Used Primer3Plus web tool to design primers for our genomic region of DPP-4. We designed 3 sets of primers which were ordered from IDT (Integrated DNA Technologies).

5/25/12 - Re-hydrated primer stocks to 100 micromolar. Made working primer solutions at 10 micromolar concentration. Autoclaved materials necessary for DNA extraction and PCR (microcentrifuge tubes, water, mineral oil, and pipet tips). Discussed project poster and created a google presentation document.

5/31/12 - Collected DNA samples from two people. Extracted DNA from cells using dneasy tissue kit (QIAGEN) following manufacturing protocols.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

Our favorite team snack is Russian sweet bread that is filled with fruit. Its called Peroshki. We also like to drink Mountain Dew and eat chips.

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 /4/12 - Figured out that DPP-4 is naturally produced in humans and in patients with Celiac disease DPP-4 maybe deficient. We are going to target DPP-4 for isolation from human cells.
+/18/12 - Trouble finding genomic sequences for DPP-4 and could only find whole genome shotgun sequences.
+/27/12 - Donut party. Team discussed other options for proteins in bacterial organisms that would breakdown casein and gluten. Team discussed the University of Washington's project in 2011 on gluten intolerance.
+/11/12 - Using NCBI genbank database in conjunction with the ensembl database we were able to figure out that DPP-4 genomic DNA was spread out over 60,000 base pairs with many large intervening regions between the coding sequences. Our team decided what our opinions were for continuing DPP-4 as our Bio-Brick. We determined the best course of actions was to amplify a 3000-4000 base pair region for DPP-4. Used Primer3Plus web tool to design primers for our genomic region of DPP-4. We designed 3 sets of primers which were ordered from IDT (Integrated DNA Technologies).
+/25/12 - Re-hydrated primer stocks to 100 micromolar. Made working primer solutions at 10 micromolar concentration. Autoclaved materials necessary for DNA extraction and PCR (microcentrifuge tubes, water, mineral oil, and pipet tips). Discussed project poster and created a google presentation document.
+/31/12 - Collected DNA samples from two people. Extracted DNA from cells using dneasy tissue kit (QIAGEN) following manufacturing protocols.
 ===Results/Conclusions===