Team:Heidelberg LSL/Parts

From 2012hs.igem.org

(Difference between revisions)

Latest revision as of 03:05, 17 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Note: All parts were carefully characterized before submission to the registry. Details about the characterization can be found in our Measurement Section or directly on the different parts pages in the registry.

Name	Description	Registry link	Part type	Availability
precA_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).	BBa_K862000	Measurement	parts registry
psulA_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).	BBa_K862001	Measurement	parts registry
precB_LacZ	This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).	BBa_K862002	Measurement	parts registry
precB	The RecB promoter sequence was obtained from the E.coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part. RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'	BBa_K862003	Regulatory	can be cloned on oligo basis (sequence provided)

@@ Line 28: / Line 28: @@
 <tr>
-<td>precA_LacZ<br/></td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).</td><td>
+<td>precA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/c/c0/BBa_K862000.PNG"/><br/>
+<br/>
+This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (<a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td>
 <p>
@@ Line 34: / Line 36: @@
   </p>
-</td><td> Measurement</td><td>partsregistry</td></tr>
+</td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 <tr>
-<td>psulA_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
+<td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/>
+<br/>
+This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (<a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
-</td><td>partsregistry</td></tr>
+</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 <tr>
-<td>precB_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
+<td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/>
+<br/>
+This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (<a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
-</td><td>partsregistry</td></tr>
+</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 </td></tr>
 <tr>
 <td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
-The recB promoter sequence was taken for the <i>E. coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
+<br/>
-We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part.
+The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
+We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.
 <br>
 <br/>RecB_fw:	5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br>