Team:Tyngsboro MA Tigers/Safety

From 2012hs.igem.org

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== Safety ==
== Safety ==
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Each member of the Tyngsborough iGEM Tiger Squad has learned and currently follows the Flynn's scientific safety contract. Each year, we take a scientific safety refresher to brush up on our safety procedures.
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Each member of the Tyngsborough iGEM Tiger Squad has learned and currently follows the Flinn's scientific safety contract. Each year, we take a scientific safety refresher to brush up on our safety procedures.
We were always supervised in our labs by our Team Leader, Ms. Ravgiala.
We were always supervised in our labs by our Team Leader, Ms. Ravgiala.
-
Click here for a [http://www.youtube.com/watch?v=GOQAq4Nl4og link to a funny Youtube video] of what NOT to do in a lab setting.  (All credit goes to the producer of the video Zach Lipovsky.)
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'''Environmental Dangers'''<br>
 +
With our device, the largest environmental concern is proper disposal of the bacteria themselves and the chemicals we were using. As we were never able to test our theoretical device, the concerns surrounding the use of carbon monoxide gas were not an issue.  We were always careful to properly dispose of cell growth plates and microtubes, always letting them sit for 20 minutes in 10% bleach and sealing them properly.  Chemicals that could be possibly considered dangerous were always used in a waste hood and disposed of properly in designated chemical waste containers.
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Click here for the [https://2012hs.igem.org/File:Safety_Contract-1.pdf Safety Contract] read and signed by all students.
+
'''Personal Safety Procedures'''<br>
 +
Our whole team was always supervised by an instructor, and wore gloves, goggles, and aprons.  We used different antibiotics in our transformations, such as ampicillin, and were always very careful and handled them in the chemical safety hood.
-
'''Environmental Dangers'''
+
'''Sterile Handling and Aseptic Techniques'''<br>
-
With our device, the largest environmental concern is proper disposal of the bacteria themselves and the chemicals we were using.  As we were never able to test our theoretical device, the concerns of using carbon monoxide were not a concern.  We were always careful to properly dispose of cell growth plates and micro tubes, always letting them seit for 20 minutes in 10% bleach and sealing them properly.  Chemicals that could be possibly considered dangerous were always used in a waste hood and disposed of properly.
+
When using the bacteria, we never re-used pipette tips or other transferal equipment that had a chance of contaminating the experiment.  We wore gloves at all times when handling bacteria, and were certain to clean all equipment before and after use and kept all equipment sterile during lab procedures.  All materials, whether they were reusable or being thrown out, were cleaned thoroughly and with bleach when applicable.
-
'''Personal Injury Dangers'''
+
<b>Lab Safety Extras</b>
 +
Click here for a [http://www.youtube.com/watch?v=GOQAq4Nl4og link to a funny Youtube video] of what NOT to do in a lab setting.  (All credit goes to the producer of the video Zach Lipovsky.)
-
Aseptic Technique
+
Click here for the [https://2012hs.igem.org/File:Safety_Contract-1.pdf Safety Contract] read and signed by all students.
 +
== Demonstrating Safety At iGEM ==
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Lab Sanitation
+
'''Ethanol'''
 +
''Before every lab, the lab tables are wiped down
 +
using 70% ethanol and paper towels.''
 +
[[File:956.jpg]]
 +
'''Sterilized Materials'''
 +
''The cup contains bleach, which is where spreaders, pipet tips
 +
and other used materials are put to safely dispose of them.''
 +
[[File:942.jpg]]
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However, given the rather unusual implications of having a synthetic biology club in a high school, we also took many other precautions against harming us and the environment.  We always were very careful handling the genetic material, and wore gloves and goggles during any experiments, especially ones involving bacteria.  All equipment used, whether it was thrown away or reusable, was cleaned in bleach thoroughly after each time we used it.
 
-
For environmental considerations of our 'machine,' we found no harmful implications.  The use of the machine would require no direct contact with the E. coli on the part of the user, and the genetic parts we were combining have no foreseeable negative effects on humans or the environment, using only detectors and the banana scent producing part already discovered and used by MIT.
+
'''Using New Pipet Tips'''
-
== Demonstrating Safety At iGEM ==
+
''Each pipet tip is only used once, and then is put in bleach.
-
[[File:956.jpg]]
+
New pipet tips are taken from the sterile container.''
-
 
+
-
[[File:942.jpg]]
+
[[File:950.jpg]]
[[File:950.jpg]]
 +
 +
 +
'''Personal Safety Precautions'''
 +
 +
''When doing a lab, every team member wears gloves and goggles.''
[[File:952.jpg]]
[[File:952.jpg]]

Latest revision as of 19:07, 15 June 2012

Screen_shot_2012-06-14_at_10.46.17_PM.png



Safety

Each member of the Tyngsborough iGEM Tiger Squad has learned and currently follows the Flinn's scientific safety contract. Each year, we take a scientific safety refresher to brush up on our safety procedures. We were always supervised in our labs by our Team Leader, Ms. Ravgiala.

Environmental Dangers
With our device, the largest environmental concern is proper disposal of the bacteria themselves and the chemicals we were using. As we were never able to test our theoretical device, the concerns surrounding the use of carbon monoxide gas were not an issue. We were always careful to properly dispose of cell growth plates and microtubes, always letting them sit for 20 minutes in 10% bleach and sealing them properly. Chemicals that could be possibly considered dangerous were always used in a waste hood and disposed of properly in designated chemical waste containers.

Personal Safety Procedures
Our whole team was always supervised by an instructor, and wore gloves, goggles, and aprons. We used different antibiotics in our transformations, such as ampicillin, and were always very careful and handled them in the chemical safety hood.

Sterile Handling and Aseptic Techniques
When using the bacteria, we never re-used pipette tips or other transferal equipment that had a chance of contaminating the experiment. We wore gloves at all times when handling bacteria, and were certain to clean all equipment before and after use and kept all equipment sterile during lab procedures. All materials, whether they were reusable or being thrown out, were cleaned thoroughly and with bleach when applicable.

Lab Safety Extras

Click here for a [http://www.youtube.com/watch?v=GOQAq4Nl4og link to a funny Youtube video] of what NOT to do in a lab setting. (All credit goes to the producer of the video Zach Lipovsky.)

Click here for the Safety Contract read and signed by all students.

Demonstrating Safety At iGEM

Ethanol

Before every lab, the lab tables are wiped down using 70% ethanol and paper towels.

956.jpg


Sterilized Materials

The cup contains bleach, which is where spreaders, pipet tips

and other used materials are put to safely dispose of them.

942.jpg


Using New Pipet Tips

Each pipet tip is only used once, and then is put in bleach.

New pipet tips are taken from the sterile container.

950.jpg


Personal Safety Precautions

When doing a lab, every team member wears gloves and goggles.

952.jpg


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