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Team:Heidelberg LSL/Parts
From 2012hs.igem.org
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| - | <td>precA_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It | + | <td>precA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/c/c0/BBa_K862000.PNG"/><br/> |
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| + | This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (<a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td> | ||
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| - | </td><td> Measurement</td><td>partsregistry</td></tr> | + | </td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr> |
<tr> | <tr> | ||
| - | <td>psulA_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It | + | <td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/> |
| + | <br/> | ||
| + | This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (<a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement | ||
| - | </td><td>partsregistry</td></tr> | + | </td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr> |
<tr> | <tr> | ||
| - | <td>precB_LacZ</td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It | + | <td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/> |
| + | <br/> | ||
| + | This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (<a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement | ||
| - | </td><td>partsregistry</td></tr> | + | </td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr> |
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
| - | <td>precB</td><td>The | + | <td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/> |
| - | We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI | + | <br/> |
| + | The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. | ||
| + | We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part. | ||
<br> | <br> | ||
<br/>RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br> | <br/>RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br> | ||
Latest revision as of 03:05, 17 June 2012
| Note: All parts were carefully characterized before submission to the registry. Details about the characterization can be found in our Measurement Section or directly on the different parts pages in the registry. |
| Name | Description | Registry link | Part type | Availability |
| precA_LacZ |  This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004). | Measurement | parts registry | |
| psulA_LacZ |  This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004). | BBa_K862001 | Measurement | parts registry |
| precB_LacZ |  This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004). | BBa_K862002 | Measurement | parts registry |
| precB |  The RecB promoter sequence was obtained from the E.coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part. RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3' | BBa_K862003 | Regulatory | can be cloned on oligo basis (sequence provided) |
