Team:Heidelberg LSL/Parts

From 2012hs.igem.org

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<a name="UV_and_radioactive_radiation_measurement_parts"></a><h2> <span class="mw-headline">Main Measurement Constructs - Engineered</span></h2>
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<td> <b> Note: All parts were carefully characterized before submission to the registry. Details about the characterization can be found in our <b> <a href="https://2012hs.igem.org/Team:Heidelberg_LSL/Measurement">Measurement Section</a> </b> or directly on the different parts pages in the registry. </b></td></tr>
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<td width="100px"> Name</td><td width="500px">Description</td><td width="100px">Registry link</td><td width="100px">physical DNA send
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<td> Name</td><td>Description</td><td>Registry link</td><td>Part type</td><td>Availability</td></tr>
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<td>precA_LacZ</td><td>BGH(rc)/shRNA10(rc)/RSV(rc)/CMV/Luc2_sv40/CMV/Kozag_hRluc_BGH</td><td>
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<td>precA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/c/c0/BBa_K862000.PNG"/><br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (<a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td>
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</td><td> pSMB_miTuner Plasmid HD3
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</td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
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<td>K4</td><td>BGH(rc)/shRNA10(rc)/RSV(rc)/CMV_TetO2/Luc2_sv40/CMV/Kozag_hRluc_BGH</td><td><a href="http://partsregistry.org/Part:BBa_K337038" class="external text" title="http://partsregistry.org/Part:BBa_K337038" rel="nofollow">BBa_K337038</a></td><td> pSMB_miTuner Plasmid HD4
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<td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (<a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
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<td>miM</td><td>sv40ter(rc)/eBFP(rc)/biCMV/eGFP(fw)/sv40ter(fw)</td><td><a href="http://partsregistry.org/Part:BBa_K337049" class="external text" title="http://partsregistry.org/Part:BBa_K337049" rel="nofollow">BBa_K337049</a></td><td> pSMB_miMeasure
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<td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (<a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
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<td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
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The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
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We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.
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<br/>RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br>
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<br>RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC</br>GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'</td><td><a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003" rel="nofollow">BBa_K862003</a></td><td> Regulatory
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</td><td>can be cloned on oligo basis (sequence provided)</td></tr>
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Latest revision as of 03:05, 17 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg




Note: All parts were carefully characterized before submission to the registry. Details about the characterization can be found in our Measurement Section or directly on the different parts pages in the registry.

NameDescriptionRegistry linkPart typeAvailability
precA_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).

BBa_K862000

Measurement parts registry
psulA_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004). 		BBa_K862001 Measurement parts registry
precB_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).		BBa_K862002 Measurement parts registry
precB

The RecB promoter sequence was obtained from the E.coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.

RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA
CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3'

RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC
GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'		BBa_K862003 Regulatory can be cloned on oligo basis (sequence provided)

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