Team:Heidelberg LSL/Notebook-24/02/2012
From 2012hs.igem.org
(Difference between revisions)
Sholderbach (Talk | contribs) |
|||
(3 intermediate revisions not shown) | |||
Line 19: | Line 19: | ||
<!--Bitte ab hier den Inhalt hineinschreiben--> | <!--Bitte ab hier den Inhalt hineinschreiben--> | ||
+ | *Inoculation of overnight cultures by picking one single colony from the transformed plates and adding it to 5 ml LB-Amp in a 50 ml falcon tube. The culture is incubated at 37 °C at 180 rpm. | ||
+ | <br/> | ||
+ | *Inoculation of LacZ (BBa_K173004) that we got from the registry through the Sourjik Lab on campus as agar stick into 5 ml LB Amp overnight culture | ||
<!--Bitte den Inhalt nur bis hier und nicht unterhalb dieser Zeile hineinschreiben--> | <!--Bitte den Inhalt nur bis hier und nicht unterhalb dieser Zeile hineinschreiben--> | ||
{{NotebookLower}} | {{NotebookLower}} |
Latest revision as of 19:14, 6 June 2012
Notebook
Welcome to our notebook!
Here you will find the documentation of our laboratory work of the last few month in diary form. This notebook comprises the work in three phases:
|
|
|
Biosensor Construction - 24/02/2012
- Inoculation of overnight cultures by picking one single colony from the transformed plates and adding it to 5 ml LB-Amp in a 50 ml falcon tube. The culture is incubated at 37 °C at 180 rpm.
- Inoculation of LacZ (BBa_K173004) that we got from the registry through the Sourjik Lab on campus as agar stick into 5 ml LB Amp overnight culture