Team:NC School of Sci Math

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<li><a href="https://2012hs.igem.org/Team:NC_School_of_Sci_Math/notebooks" accesskey="5" title="">Lab Notebook</a></li>
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<h2 class="title"><a href="#">Welcome to the NCSSM iGEM Wiki</a></h2>
<h2 class="title"><a href="#">Welcome to the NCSSM iGEM Wiki</a></h2>
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<h2>NCSSM</h2>
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<p>The North Carolina School of Science and Mathematics is residential public high school for highly gifted 11th and 12th graders in the state of North Carolina. Students at NCSSM regularly take advantage of the schools research opportunities. NCSSM iGEM is the latest research opportunity to these students!</p>
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<h2>Useful Links</h2>
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<li><a href="http://www.ncssm.edu/">NCSSM</a></li>
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<li><a href="http://www.synberc.org/">SynBERC</a></li>
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<li><a href="http://pubs.acs.org/journal/asbcd6">ACS Synethetic Biology</a></li>
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===Abstract===
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Silver nanoparticles (AgNP’s) have a variety of beneficial health effects but can be detrimental to both human and environmental health in excessive quantities. As a result, NCSSM iGEM has attempted to develop an inexpensive biosensor for detecting AgNP’s in vivo. This novel AgNP biosensor utilizes ACE1, a silver binding transcription factors from S. cerevisiae (yeast), in an E. coli chassis. In the biosensor’s synthetic gene network, a constitutive promoter upstream of ACE1 ensures continuous expression of the ACE1 protein. In the presence of AgNP’s, ACE1 activates the CUP1 promoter which has been placed upstream of a Green Fluorescent Protein gene. Subsequently, exposure of the E. coli biosensor to AgNP’s will result in high levels of GFP expression. Network success was confirmed in vivo by spreading the biosensor on plates containing varying concentrations of AgNP’s. However, due to the antimicrobial effects of AgNP’s, network success under high AgNP concentrations remains unconfirmed.

Latest revision as of 02:27, 17 June 2012

Welcome to the NCSSM iGEM Wiki

Abstract

Silver nanoparticles (AgNP’s) have a variety of beneficial health effects but can be detrimental to both human and environmental health in excessive quantities. As a result, NCSSM iGEM has attempted to develop an inexpensive biosensor for detecting AgNP’s in vivo. This novel AgNP biosensor utilizes ACE1, a silver binding transcription factors from S. cerevisiae (yeast), in an E. coli chassis. In the biosensor’s synthetic gene network, a constitutive promoter upstream of ACE1 ensures continuous expression of the ACE1 protein. In the presence of AgNP’s, ACE1 activates the CUP1 promoter which has been placed upstream of a Green Fluorescent Protein gene. Subsequently, exposure of the E. coli biosensor to AgNP’s will result in high levels of GFP expression. Network success was confirmed in vivo by spreading the biosensor on plates containing varying concentrations of AgNP’s. However, due to the antimicrobial effects of AgNP’s, network success under high AgNP concentrations remains unconfirmed.

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