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Team:Heidelberg LSL/Notebook materials
From 2012hs.igem.org
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| - | <a http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/t4-dna-ligase/">T4 DNA ligase | + | <a href="http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/t4-dna-ligase/">T4 DNA ligase |
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</p></td><td>Ligation of DNA fragments</td><td>Promega or Fermentas</td> | </p></td><td>Ligation of DNA fragments</td><td>Promega or Fermentas</td> | ||
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| - | <a http://www.fermentas.com/en/products/all/pcr-qpcr-rt-pcr/standard-pcr/k017-pcr-master-mix7">2x PCR MasterMix | + | <a href="http://www.fermentas.com/en/products/all/pcr-qpcr-rt-pcr/standard-pcr/k017-pcr-master-mix7">2x PCR MasterMix |
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</p></td><td>Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screens</td><td>Fermentas</td> | </p></td><td>Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screens</td><td>Fermentas</td> | ||
Revision as of 13:52, 11 June 2012
Kits for Plasmid and DNA purification
| Name | Product Description | Company |
| small scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains | Promega | |
| small scale plasmid purification from E. coli Top10 cells before sequencing | Qiagen | |
| medium scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains | Promega | |
| direct purification of DNA after restriction digests | Qiagen | |
| Purification of DNA fragements from agarose gel | Qiagen |
Enzymes
| Name | Product Description | Company |
| restriction enzyme, recognition sequence: GAATTC | Promega or NEB | |
| restriction enzyme, recognition sequence: ACTAGT | Promega or NEB | |
| restriction enzyme, recognition sequence: TCTAGA | Promega or NEB | |
| restriction enyzme, recognition sequence: CTGCAG | Promega or NEB | |
| Ligation of DNA fragments | Promega or Fermentas | |
| Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screens | Fermentas |
