Team:Heidelberg LSL/Notebook materials

From 2012hs.igem.org

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<h4>Enzymes and Polymerase</h4>
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<td> Name</td><td>Product Description</td><td>Company</td></tr>
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  <a href="http://www.promega.com/products/cloning-and-dna-markers/restriction-enzymes/ecori/">EcoRI
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</p></td><td>restriction enzyme, recognition sequence: GAATTC </td><td>Promega or NEB</td>
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  <a href="http://www.promega.com/products/cloning-and-dna-markers/restriction-enzymes/spei/">SpeI
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</a>
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</p></td><td>restriction enzyme, recognition sequence: ACTAGT </td><td>Promega or NEB</td>
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<td><p>
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  <a href="http://www.promega.com/products/cloning-and-dna-markers/restriction-enzymes/xbai/">PureYield™ Plasmid Midiprep System
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</a>
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</p></td><td>restriction enzyme, recognition sequence: TCTAGA</td><td>Promega or NEB</td>
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<td><p>
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  <a href="http://www.promega.com/products/cloning-and-dna-markers/restriction-enzymes/psti/">PstI
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</a>
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</p></td><td>restriction enyzme, recognition sequence: CTGCAG</td><td>Promega or NEB</td>
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<td><p>
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  <a http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/t4-dna-ligase/">T4 DNA ligase
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</p></td><td>Ligation of DNA fragments</td><td>Promega or Fermentas</td>
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  <a http://www.fermentas.com/en/products/all/pcr-qpcr-rt-pcr/standard-pcr/k017-pcr-master-mix">2x PCR MasterMix
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</p></td><td>Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screens</td><td>Fermentas</td>
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<td><p>
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  <a href="http://www.qiagen.com/dm/aw/miniprep/default.aspx?gaw=QVenGA3Miniprep&gkw=miniprep+kit">QIAprep Spin MiniPrep Kit (50)
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</a>
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</p></td><td>small scale plasmid purification from E. coli Top10 cells before sequencing </td><td>Qiagen</td>
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Revision as of 13:49, 11 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Kits for Plasmid and DNA purification

NameProduct DescriptionCompany

PureYield™ Plasmid Miniprep System

small scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains Promega

QIAprep Spin MiniPrep Kit (50)

small scale plasmid purification from E. coli Top10 cells before sequencing Qiagen

PureYield™ Plasmid Midiprep System

medium scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains Promega

QIAquick Nucleotide Removal Kit

direct purification of DNA after restriction digestsQiagen

QIAquick Gel Extraction Kit (50)

Purification of DNA fragements from agarose gelQiagen

Enzymes and Polymerase

Retrieved from "http://2012hs.igem.org/Team:Heidelberg_LSL/Notebook_materials"
NameProduct DescriptionCompany

EcoRI

restriction enzyme, recognition sequence: GAATTC Promega or NEB

SpeI

restriction enzyme, recognition sequence: ACTAGT Promega or NEB

PureYield™ Plasmid Midiprep System

restriction enzyme, recognition sequence: TCTAGAPromega or NEB

PstI

restriction enyzme, recognition sequence: CTGCAGPromega or NEB

T4 DNA ligase

Ligation of DNA fragmentsPromega or Fermentas

2x PCR MasterMix

Standard Taq PCR master mix for robust amplification of DNA-Fragments by PCR; used for colony-PCR screensFermentas

QIAprep Spin MiniPrep Kit (50)

small scale plasmid purification from E. coli Top10 cells before sequencing Qiagen