Team:Heidelberg LSL/Notebook materials

From 2012hs.igem.org

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<h4>Kits for Plasmid and DNA purification</h4>
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  <a href="http://www.promega.com/b/de/aufreinigung/minis/default.aspx">PureYield™ Plasmid Miniprep System
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</p></td><td>small scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains </td><td>Promega</td>
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  <a href="http://www.qiagen.com/dm/aw/miniprep/default.aspx?gaw=QVenGA3Miniprep&gkw=miniprep+kit">QIAprep Spin MiniPrep Kit (50)
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</p></td><td>small scale plasmid purification from E. coli Top10 cells before sequencing </td><td>Qiagen</td>
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  <a href="http://www.promega.com/products/dna-and-rna-purification/plasmid-purification/pureyield-plasmid-midiprep-system/">PureYield™ Plasmid Midiprep System
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</p></td><td>medium scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains </td><td>Promega</td>
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  <a href="http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquicknucleotideremovalkit.aspx">QIAquick Nucleotide Removal Kit
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</p></td><td>direct purification of DNA after restriction digests</td><td>Qiagen</td>
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   <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>  
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   <a href="http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickgelextractionkit.aspx">QIAquick Gel Extraction Kit (50)
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  </p></td><td>This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).</td>
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  </p></td><td>Purification of DNA fragements from agarose gel</td><td>Qiagen</td>
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Revision as of 13:34, 11 June 2012

iGEM-2012HS - LSL-Heidelberg iGEM-2012HS - LSL-Heidelberg

Kits for Plasmid and DNA purification

NameProduct DescriptionCompany

PureYield™ Plasmid Miniprep System

small scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains Promega

QIAprep Spin MiniPrep Kit (50)

small scale plasmid purification from E. coli Top10 cells before sequencing Qiagen

PureYield™ Plasmid Midiprep System

medium scale plasmid purification from E. coli Top10 cells before digestion or transformation into different E. coli strains Promega

QIAquick Nucleotide Removal Kit

direct purification of DNA after restriction digestsQiagen

QIAquick Gel Extraction Kit (50)

Purification of DNA fragements from agarose gelQiagen